Premise of the study: New sequencing technologies enable the possibility of generating large-scale molecular datasets for constructing the plant tree of life. We describe a new probe set for target enrichment sequencing to generate nuclear sequence data to build phylogenetic trees with any flagellate plants, comprising hornworts, liverworts, mosses, lycophytes, ferns, and gymnosperms. Methods and Results: We leveraged existing transcriptome and genome sequence data to design a set of 56,989 probes for target enrichment sequencing of 451 nuclear exons and non-coding flanking regions across flagellate plant lineages. We describe the performance of target enrichment using the probe set across flagellate plants and demonstrate the potential of the data to resolve relationships among both ancient and closely related taxa. Conclusions: A target enrichment approach using the new probe set provides a relatively low-cost solution to obtain large-scale nuclear sequence data for inferring phylogenetic relationships across flagellate plants.

中文翻译：

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用于解决鞭毛植物生命树的目标富集探针组

研究的前提：新的测序技术使生成构建植物生命树的大规模分子数据集成为可能。我们描述了一种用于目标富集测序的新探针集，以生成核序列数据，以建立与任何鞭毛植物的系统发育树，包括鞭毛，肝苔，苔藓，苔藓植物，蕨类植物和裸子植物。方法和结果：我们利用现有的转录组和基因组序列数据设计了一套56,989个探针，用于鞭毛植物谱系中451个核外显子和非编码侧翼区域的靶标富集测序。我们描述了使用跨鞭毛植物探针集的目标富集的性能，并展示了数据解决古老和密切相关的分类群之间关系的潜力。结论：