We developed a panel of 44 nuclear genetic markers and applied this to two species of marbled newts in the north (Triturus marmoratus) and the south (T. pygmaeus) of the Iberian Peninsula, to document pattern and process of interspecific gene flow. The northernmost occurrence of T. pygmaeus genetic material was in a T. marmoratus population north of the Vouga river estuary. This suggested the past presence of a hybrid zone, possibly coinciding with a natural river outlet at ca. 1200 A.D. Since 1808, the species contact has moved back south to a by then completed, man-made Vouga channel. We also found evidence for a T. marmoratus genomic footprint in T. pygmaeus from the Serra de Sintra, near Lisbon. In combination with a previously reported southern, relic occurrence of T. marmoratus in between both areas, the data point to the superseding with hybridization of T. marmoratus by T. pygmaeus. We estimate that the species hybrid zone has moved over a distance of ca. 215 km.

中文翻译：

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大理石new中移动混合带的基因组足迹

我们开发了一个由44个核遗传标记组成的小组，并将其应用于伊比利亚半岛北部（Triturus marmoratus）和南部（T. pygmaeus）的两种大理石new，以记录种间基因流动的方式和过程。pygmaeus遗传物质的最北端出现在Vouga河口以北的marmoratus种群中。这表明过去存在着一个混合带，可能恰好与加利福尼亚州的自然河流出口相吻合。公元1200年自1808年以来，物种接触已向南移至一条已建成的人造Vouga通道。我们还从里斯本附近的Serra de Sintra的pygmaeus中发现了T. marmoratus基因组足迹。结合先前报道的在两个地区之间南部出现的玛氏锥虫遗迹，数据表明，pygmaeus取代了Marmoratus杂交。我们估计，物种混合区已经移动了约200米的距离。215公里