We report a novel scaffold for clustering and oriented immobilization of human IgG1 Fc-fused lectins on biosensors without chemical modifications. This approach uses a bio-nanocapsule (BNC) displaying a tandem form of IgG Fc-binding Z domains derived from Staphylococcus aureus protein A (ZZ-BNC). Incorporating ZZ-BNC effectively increased both the sensitivity and sugar chain-binding capacity compared with the condition without ZZ-BNC.

中文翻译：

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通过定向固定Fc融合的凝集素来增强糖链检测

我们报告了一种新型支架，用于在无需化学修饰的生物传感器上对人IgG1 Fc融合凝集素进行聚类和定向固定。此方法使用生物纳米球（BNC），其显示串联形式的源自金黄色葡萄球菌蛋白A（ZZ-BNC）的IgG Fc结合Z结构域。与没有ZZ-BNC的条件相比，掺入ZZ-BNC可以有效地提高敏感性和糖链结合能力。