The assembly of human cytomegalovirus (HCMV) virions is an orchestrated process that requires, as an essential prerequisite, the complex crosstalk between viral structural proteins. Currently, however, the mechanisms governing the successive steps in the constitution of virion protein complexes remain elusive. Protein phosphorylation is a key regulator determining the sequential changes in the conformation, binding, dynamics, and stability of proteins in the course of multiprotein assembly. In this review, we present a comprehensive map of the HCMV virion proteome, including a refined view on the virion phosphoproteome, based on previous publications supplemented by new results. Thus, a novel dataset of viral and cellular proteins contained in HCMV virions is generated, providing a basis for future analyses of individual phosphorylation steps and sites involved in the orchestrated assembly of HCMV virion-specific multiprotein complexes. Finally, we present the current knowledge on the activity of pUL97, the HCMV-encoded and virion-associated kinase, in phosphorylating viral and host proteins.

中文翻译：

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基于质谱的人巨细胞病毒病毒体蛋白质组，磷酸化蛋白质组和相关激酶活性的表征。

人类巨细胞病毒（HCMV）病毒体的组装是一个精心策划的过程，需要一个基本的前提条件，即病毒结构蛋白之间的复杂串扰。然而，目前，控制病毒体蛋白复合物的连续步骤的机制仍然难以捉摸。蛋白质磷酸化是决定多蛋白组装过程中蛋白质构象，结合，动力学和稳定性的顺序变化的关键调节剂。在这篇综述中，我们基于以前的出版物和新结果，提供了HCMV病毒体蛋白质组的全面图谱，包括对病毒体磷酸化蛋白质组的细化视图。因此，生成了HCMV病毒体中病毒和细胞蛋白的新数据集，为将来分析HCMV病毒体特异性多蛋白复合物的精心组装中涉及的各个磷酸化步骤和位点提供基础。最后，我们介绍了有关pUL97（HCMV编码和病毒体相关激酶）在磷酸化病毒和宿主蛋白中的活性的最新知识。