The ribosome is a large ribonucleoprotein complex that synthesizes protein in all living organisms. Ribosome biogenesis is a complex process that requires synchronization of various cellular events, including ribosomal RNA (rRNA) transcription, ribosome assembly, and processing and post-transcriptional modification of rRNA. Ribosome biogenesis is fine-tuned with various assembly factors, possibly including nucleotide modification enzymes. Ribosomal small subunit pseudouridine synthase A (RsuA) pseudouridylates U516 of 16S helix 18. Protein RsuA is a multi-domain protein that contains the N-terminal peripheral domain, which is structurally similar to the ribosomal protein S4. Our study shows RsuA preferably binds and pseudouridylates an assembly intermediate that is stabilized by ribosomal protein S17 over the native-like complex. In addition, the N-terminal domain truncated RsuA showed that the presence of the S4-like domain is important for RsuA substrate recognition.

中文翻译：

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假尿苷合酶RsuA捕获由核糖体蛋白S17稳定的装配中间体。

的 核糖体是一种大型的核糖核蛋白复合物，可以在所有活生物体中合成蛋白质。核糖体生物发生是一个复杂的过程，需要同步各种细胞事件，包括核糖体RNA（rRNA）转录，核糖体组装以及rRNA的加工和转录后修饰。核糖体的生物发生可通过各种装配因子（包括核苷酸修饰酶）进行微调。核糖体小亚基假尿苷合酶A（RsuA）假尿苷化16S螺旋18的U516。蛋白RsuA是包含N末端外围结构域的多结构域蛋白，其结构类似于核糖体蛋白S4。我们的研究表明，RsuA最好结合并假尿苷化由核糖体蛋白S17稳定于天然样复合物上的装配中间体。此外，