A variety of biosensing strains for monitoring of aromatic hydrocarbon pollutants have been developed but only a few have been applied to the field due to their lower survival rate and susceptibility to toxic pollutants. Immobilization is one of the solutions to protect strain from toxic pollutants. Synthetic and natural matrices were screened for the immobilization of three strains, which were able to detect one‐, two‐ and three‐ring aromatic hydrocarbon compounds. Agarose and calcium alginate were found to be the best matrices for strain immobilization. The survival rate of immobilized strains was determined by the colony‐forming unit (CFU) count at regular intervals till it reaches to the non‐detectable level. The stability of immobilized biosensing strains was found to be 12–15 days in the industrial wastewater. Hence vector from strains was transformed into wastewater isolate Pseudomonas citronellolis. Immobilized Pseudomonas citronellolis was stable for 66 days in industrial wastewater. Immobilized strains may be applied to design automated biosensor for the online monitoring of pollutants.

中文翻译：

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工业废水中芳烃检测用固定化工艺的优化和微生物传感菌株的存活研究

已经开发了用于监测芳族烃污染物的多种生物传感菌株，但是由于它们的较低的存活率和对有毒污染物的敏感性，因此仅在该领域中应用了少数。固定化是保护菌株免受有毒污染物污染的解决方案之一。筛选了合成和天然基质以固定三种菌株，这些菌株能够检测一环，二环和三环芳烃化合物。琼脂糖和海藻酸钙被发现是固定菌株的最佳基质。固定的菌株的存活率由菌落形成单位（CFU）定期计数直至达到不可检测的水平来确定。在工业废水中，固定化的生物传感菌株的稳定性为12-15天。香茅假单胞菌。固定的香茅假单胞菌在工业废水中稳定66天。固定的菌株可以用于设计自动化的生物传感器，用于污染物的在线监测。