This study investigated the effects of BMP‐15 on the in vitro development of preantral follicles of collared peccaries. Ovarian fragments were cultured for 1 or 6 days in Tissue Culture Medium 199 (TCM199⁺) supplemented with BMP‐15 at rates of 0, 1, 25 or 50 ng/ml. The fragments were analysed histologically by evaluating follicular morphology, activation and growth as well as the potential for proliferation of granulosa cells. Our results show the addition of 25 ng/ml BMP‐15 in the medium provided the greatest percentage of normal follicles (79.67% ± 0.69) when compared to other treatments (p < .05); however, this result is similar to 1 ng/ml BMP‐15 (74.00% ± 1.90, p > .05). Moreover, 25 and 50 ng/ml of BMP‐15 promoted follicular activation. BMP‐15 supplements did not affect oocyte and follicular growth. All concentrations of BMP‐15 increased the number of nucleolus organizer regions (NORs) after 1 day of culture when compared to fresh fragments or the control samples (p < .05). However, at the end of the experiment, the number of NORs in follicles cultured in all treatments was higher than that observed in the fresh control (sample taken prior to culturing) (p > .05). In summary, the addition of 25 ng/ml BMP‐15 to the culture medium of collared peccary preantral follicles maintained a high number of morphologically healthy follicles and stimulated the activation of primordial follicles after 6 days in culture.

中文翻译：

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BMP-15活性对颈项腔（Pecari tajacu Linnaeus，1758）腔前卵泡的体外发育。

这项研究调查了BMP-15对衣领腹腔前腔卵泡体外发育的影响。在补充了BMP-15的组织培养基199（TCM199 ⁺）中以0、1、25或50 ng / ml的速率将卵巢片段培养1或6天。通过评估滤泡形态，活化和生长以及颗粒细胞增殖的潜力，对这些片段进行组织学分析。我们的结果表明，与其他治疗相比，在培养基中添加25 ng / ml BMP-15可以提供最大比例的正常卵泡（79.67％±0.69）（p  <.05）；但是，此结果类似于1 ng / ml BMP-15（74.00％±1.90，p > .05）。此外，25和50 ng / ml的BMP-15促​​进卵泡活化。BMP-15补充剂不会影响卵母细胞和卵泡的生长。与新鲜片段或对照样品相比，培养1天后所有浓度的BMP-15均可增加核仁组织区（NORs）的数量（p  <.05）。然而，在实验结束时，在所有处理中培养的卵泡中的NOR数均高于新鲜对照（培养前采集的样本）中观察到的NOR数（p  > .05）。总而言之，在培养的第6天后，在领口的颊部腔前卵泡培养基中添加25 ng / ml BMP-15可以保持大量形态健康的卵泡，并刺激原始卵泡的活化。