1,3‐Dioleoyl‐2‐palmitoylglycerol (OPO) is the main triacylglycerol species in human milk‐fat substitute. The production of OPO is of considerable research interest. In this study, a new strategy for producing OPO by fermentation with R. opacus is proposed. Chemically Interesterified fat (high oleic acid sunflower oil/hydrogenated palm oil 1.73:1 w/w), or a mixture of ethyl oleate/ethyl palmitate 2:1 (w/w) as a starting material. The highest biomass and oil content obtained were 3.3 g L⁻¹ and 40.2% (dry cell weight), respectively. The yield of OPO was 0.62 g L⁻¹. The fatty acid composition of produced OPO was 55.7–59.7% 18:1 and 28.3–29.8% 16:0, and the sn ‐2 position was predominantly 16:0 (64.7–74.5%). ¹³C‐nuclear magnetic resonance analysis showed that the sn ‐1,3 and sn ‐2 positions were predominately esterified by 18:1 and 16:0, respectively. OPO (47.1%), OPL (13.9%), PPO (13.1%), and PPoO (16:0–16:1–18:1) (10.3%) were the most abundant triacylglycerol species.

中文翻译：

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通过不透明红球菌发酵有效生产1,3-二油基-2-棕榈酰甘油

1,3-二油酰基-2-棕榈酰甘油（OPO）是人乳脂肪替代品中的主要三酰基甘油种类。OPO的生产具有相当大的研究兴趣。在这项研究中，提出了一种新的策略，可通过用不透明红球菌发酵来生产OPO 。化学酯交换的脂肪（高油酸向日葵油/氢化棕榈油1.73：1 w / w），或油酸乙酯/棕榈酸乙酯2：1（w / w）的混合物作为起始原料。获得的最高生物质和油含量分别为3.3 g L ^-1和40.2％（干细胞重量）。OPO的产量为0.62g L ^-1。产生的OPO的脂肪酸组成为18：1的55.7–59.7％和28.3–29.8％的16：0，而sn -2的位置主要为16：0（64.7–74.5％）。¹³C核磁共振分析表明，sn -1,3和sn -2位置分别被18：1和16：0酯化。OPO（47.1％），OPL（13.9％），PPO（13.1％）和PPoO（16：0–16：1–18：1）（10.3％）是最丰富的三酰基甘油。