Pigmented macrophage aggregates (MAs) are known to change under influence of various factors, such as aging, season, starvation, and/or pollution. In this study, changes in the pigment content of the MAs in the spleen of Vardar chub (Squalius vardarensis, Karaman) (n = 129) collected in spring and autumn, from three rivers with different pollution impact was examined: Zletovska River (metals), Kriva River (metals and municipal wastewater), and Bregalnica River (municipal wastewater). Collected data revealed increased relative volume and number of MAs containing hemosiderin under the influence of metals, significant in autumn (p < .05). In chub exposed to metals combined with municipal wastewater, significant increase of lipochrome accumulation in MAs in autumn, melanin in MAs in fish captured in the spring season, and number of splenic MAs containing combination of melanin and lipochrome was noted. Volumes and number of MAs containing combination of hemosiderin and lipochrome increased in spleen of fish captured in autumn from both Zletovska River and Kriva River, most likely due to the contribution of hemosiderin and lipochrome, respectively. Values measured for the various pigments in splenic MAs in fish captured from Bregalnica River, were overall closer to the values measured for fish captured from Kriva River. Notably, melanin and lipochrome are more likely to be found in fish from waters influenced by municipal wastewater (organic pollution) and hemosiderin in fish spleen from water influenced by mining activity (heavy metals pollution).

中文翻译：

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不同污染物暴露对Vardar chub脾脏中有色巨噬细胞聚集体色素含量的影响（Squalius vardarensis Karaman，1928年）。

已知色素沉着的巨噬细胞聚集体（MA）在各种因素（例如衰老，季节，饥饿和/或污染）的影响下发生变化。在这项研究中，研究了春季和秋季采集的来自三个具有不同污染影响的河流的Vardar chub（Squalius vardarensis，Karaman）（n = 129）的脾中MAs色素含量的变化：Zletovska河（金属） ，克里瓦河（金属和市政废水）和布雷加尼察河（市政废水）。收集的数据显示，在金属的影响下，含铁血黄素的MA的相对体积和数量增加，秋季显着（p<.05）。在暴露于金属与城市污水结合的中，秋季秋季MA中脂色素的积累显着增加，春季捕获的鱼中MA中的黑色素显着增加，并且含有黑色素和脂质色素组合的脾脏MA的数量显着增加。秋季从Zletovska河和Kriva河捕获的鱼的脾脏中含有含铁血黄素和脂色素结合的MA的数量和数量增加，最可能的原因分别是含铁血黄素和脂色素的贡献。从布雷加尼察河捕获的鱼的脾脏MAs中各种色素的测量值总体上接近从克里瓦河捕获的鱼的测量值。值得注意的是