Inhibitory effects of Manihot esculenta extracts on Food‐Borne pathogens and their antioxidant properties: Supercritical fluid extraction, statistical analysis, and molecular docking study,Journal of Food Process Engineering

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Inhibitory effects of Manihot esculenta extracts on Food‐Borne pathogens and their antioxidant properties: Supercritical fluid extraction, statistical analysis, and molecular docking study
Journal of Food Process Engineering ( IF 2.7 ) Pub Date : 2020-05-29 , DOI: 10.1111/jfpe.13452
Mohammad Norazmi Ahmad _{1,

2} , Nur Hidayah Mohd Azli ₁ , Hakimah Ismail ₁ , Mohammad Anwar Mohamed Iqbal ₃ , Bijarimi Mat Piah ₄ , Erna Normaya ₁

Affiliation

An alternative natural additive from Manihot esculenta has been recently discovered. Using a response surface methodology (RSM) approach, a high content of antioxidant activity based on DPPH inhibition from the plant source was found, with the optimum production conditions being 45.9°C, 4,100 psi, and 44 min for temperature, pressure, and time, respectively. The optimized extract was analyzed using gas chromatography–mass spectrometry (GC–MS), Fourier‐transform infrared spectroscopy (FTIR), and UV–Vis spectroscopy. Based on the GC–MS result, the optimized extract contains two major compounds, namely, hexadecanoic acid (40.43%) and 9‐octadecenoic acid (32.75%). The optimized extract also showed good activity in inhibiting Bacillus cereus (9.9 ± 0.17 mm) and Escherichia coli (10.7 ± 0.18 mm). To verify the activity of these two compounds toward B. cereus and E. coli, a theoretical approach with molecular docking was used. These two compounds demonstrated good binding affinity toward 2HUC and 1L8A proteins and could be isolated for use as alternative additives in the food industry.

中文翻译：

番石榴提取物对食源性病原菌的抑制作用及其抗氧化性能：超临界流体提取，统计分析和分子对接研究

最近发现了Manihot esculenta的替代天然添加剂。使用响应表面方法（RSM）的方法，发现了基于植物来源的DPPH抑制的高含量抗氧化剂活性，最佳生产条件为45.9°C，4,100 psi，温度，压力和时间为44分钟，分别。使用气相色谱-质谱（GC-MS），傅立叶变换红外光谱（FTIR）和紫外-可见光谱对最佳提取物进行分析。根据GC-MS结果，优化的提取物包含两种主要化合物，即十六烷酸（40.43％）和9-十八烯酸（32.75％）。优化的提取物在抑制蜡状芽孢杆菌（9.9±0.17 mm）和大肠杆菌（10.7±0.18毫米）。为了验证这两种化合物对蜡状芽孢杆菌和大肠杆菌的活性，使用了分子对接的理论方法。这两种化合物对2HUC和1L8A蛋白表现出良好的结合亲和力，可以分离出来用作食品工业中的替代添加剂。

更新日期：2020-05-29

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