Investigation of the effects of squalene and squalene epoxides on the homeostasis of coenzyme Q10 in rats by UPLC‐Orbitrap MS,Chemistry & Biodiversity

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Investigation of the effects of squalene and squalene epoxides on the homeostasis of coenzyme Q10 in rats by UPLC‐Orbitrap MS
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2020-07-30 , DOI: 10.1002/cbdv.202000243
Wenjing Yu ₁ , Kaiju Sun ₁ , Liying Zhang ₁ , Xilin Wan ₁ , Changbao Chen ₁ , Rui Su ₁ , Yifei Liu ₂ , Hongfeng Wang ₁ , Hongmei Yang ₁

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Squalene has been used as a dietary supplement for a long history due to its potential cancer-preventive function. However, the mechanism has not yet been investigated in detail. Therefore, the aim of this study is to see if the plasma coenzyme Q 10 (CoQ 10 ) level will be altered by gavage of squalene and oxidosqualenes to rats. In the present work, a sensitive and simple high-performance analytical method based on ultra-high-performance liquid chromatography coupled with an Orbitrap mass spectrometry (UPLC-Orbitrap-MS) was developed for the quantification of CoQ 10 in rat plasma. Coenzyme Q 9 (CoQ 9 ) was employed as the internal standard. CoQ 10 was determined after acetonitrile-mediated plasma protein precipitation using UPLC-Orbitrap-MS in negative ion mode. Intragastric administration of squalene and the two squalene epoxides into rats once daily for several days elevated the level of CoQ 10 in their plasma, but there was no significant difference between high-dose ( 286 mg/kg ) and low-dose ( 143 mg/kg ) groups. Intragastric administration of squalene once a day for 5 consecutive days and oxidosqualens once a day for 3 consecutive days is necessary for reaching the steady-state level of CoQ 10 . Our present findings indicate that squalene and oxidosqualenes may be useful for stimulating the synthesis of CoQ 10 in rats.

中文翻译：

UPLC-Orbitrap MS研究角鲨烯和角鲨烯环氧化物对大鼠辅酶Q10稳态的影响

由于其潜在的癌症预防功能，角鲨烯长期以来一直被用作膳食补充剂。然而，尚未对其机制进行详细研究。因此，本研究的目的是观察血浆辅酶 Q 10 (CoQ 10 ) 水平是否会因给大鼠灌胃角鲨烯和氧化角鲨烯而改变。在目前的工作中，开发了一种基于超高效液相色谱与 Orbitrap 质谱联用 (UPLC-Orbitrap-MS) 的灵敏且简单的高效分析方法，用于定量大鼠血浆中的 CoQ 10。辅酶Q 9 (CoQ 9 )用作内标。CoQ 10 是在乙腈介导的血浆蛋白沉淀后使用 UPLC-Orbitrap-MS 在负离子模式下测定的。每天一次给大鼠灌胃角鲨烯和两种角鲨烯环氧化物几天后，它们血浆中 CoQ 10 的水平升高，但高剂量 (286 mg/kg) 和低剂量 (143 mg/kg) 之间没有显着差异。公斤）组。连续 5 天每天一次的角鲨烯和连续 3 天每天一次的氧化角鲨烯的胃内给药对于达到 CoQ 10 的稳态水平是必要的。我们目前的研究结果表明，角鲨烯和氧化角鲨烯可能有助于刺激大鼠 CoQ 10 的合成。连续 5 天每天一次的角鲨烯和连续 3 天每天一次的氧化角鲨烯的胃内给药对于达到 CoQ 10 的稳态水平是必要的。我们目前的研究结果表明，角鲨烯和氧化角鲨烯可能有助于刺激大鼠 CoQ 10 的合成。连续 5 天每天一次的角鲨烯和连续 3 天每天一次的氧化角鲨烯的胃内给药对于达到 CoQ 10 的稳态水平是必要的。我们目前的研究结果表明，角鲨烯和氧化角鲨烯可能有助于刺激大鼠 CoQ 10 的合成。

更新日期：2020-07-30

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