Up to date, there is no information on the influence of 2,2,2-tribromoethanol (TBE; Avertin), a commonly used anaesthetic, on mice with impaired antioxidant capacity. We aimed to analyse the effect of a single dose of Avertin on anaesthesia duration time, inflammatory response, oxidative stress and collagen deposition in the large intestine of Nrf2 transcriptional knockout mice (tNrf2^−/−). The studies were performed on six-month-old female mice Nrf2^+/+ and tNrf2^−/− randomly assigned to Avertin (250 mg/kg b.w. single i.p. injection) or vehicle group. We observed a 2-fold increase in anaesthesia time and longer recovery time (p = 0.015) in tNrf2^−/− in comparison to Nrf2^+/+. However, no hepato- or nephrotoxicity was detected. Interestingly, we found severe changes in colon morphology of untreated tNrf2^-/- mice associated with colon shortening (p = 0.02) and thickening (p = 0.015). Avertin treatment caused colon damage manifested with epithelial layer damage and goblet depletion in Nrf2^+/+ mice but not in tNrf2^−/− individuals. Additionally, Avertin did not induce oxidative stress in colon tissue, but it increased leukocyte infiltration in Nrf2^+/+ mice (p = 0.02). Immunofluorescent staining also revealed enhanced deposition of collagen I and collagen III in the colon of untreated tNrf2^−/− mice. Avertin contributed to increased deposition of collagen I in Nrf2^+/+ mice but reduced deposition of collagen I and III in tNrf2^−/− individuals. In conclusion, tNrf2^−/− respond to Avertin with prolonged anaesthesia that is not associated with acute toxicity, inflammatory reaction or enhanced oxidative stress. Avertin does not impair intestine morphology in tNrf2^−/− mice but can normalise the enhanced fibrosis.

迄今为止，尚无关于常用的麻醉剂2,2,2-三溴乙醇（TBE; Avertin）对抗氧化能力受损的小鼠的影响的信息。我们旨在分析单剂量的Avertin对Nrf2转录敲除小鼠（tNrf2 ^-/-）大肠内麻醉持续时间，炎症反应，氧化应激和胶原沉积的影响。该研究在六个月大的雌性小鼠Nrf2 ^{+ / +}和tNrf2 ^-/-上随机分配给Avertin（250 mg / kg bw单次ip注射）或媒介物组进行。与Nrf2 ^{+ / +}相比，我们观察到tNrf2 ^-/-的麻醉时间增加了2倍，恢复时间更长（p = 0.015）。但是，未检测到肝毒性或肾毒性。有趣的是，我们发现未经治疗的tNrf2 ^-/-小鼠结肠形态发生了严重变化，与结肠缩短（p = 0.02）和增厚（p = 0.015）有关。在Nrf2 ^{+ / +}小鼠中，Avertin治疗引起的结肠损伤表现为上皮层损伤和杯状细胞耗竭，而在tNrf2 ^-/-个体中则没有。此外，Avertin不会在结肠组织中诱发氧化应激，但会增加Nrf2 ^{+ / +}小鼠中的白细胞浸润（p = 0.02）。免疫荧光染色还显示，未经处理的tNrf2 ^-/-小鼠结肠中胶原蛋白I和胶原蛋白III的沉积增强。Avertin促进Nrf2 ^{+ / +}中胶原蛋白I的沉积小鼠，但减少了tNrf2 ^-/-个体中的胶原蛋白I和III的沉积。总之，tNrf2 ^-/-对Avertin的反应时间长，与急性毒性，炎症反应或氧化应激增加无关。Avertin不会损害tNrf2 ^-/-小鼠的肠道形态，但可以使增强的纤维化正常化。