Breast cancer is one of the commonly occurred cancers among women and poses a huge threat against female health. Abnormal expression of lncRNA has been confirmed to be an important inducer of cancer. By searching GEO and TCGA database, we found that CENPF was upregulated in breast cancer tissues. Through RT-qPCR, CENPF was found to be upregulated in breast cancer cells. Functional experiments revealed that CENPF had positive effect on the cellular functions, including proliferation, migration and invasion. Subsequently, CENPF was confirmed to combine with miR-28-5p, and its expression was suppressed by miR-28-5p. Furthermore, it was found that miR-28-5p bound to MCM3AP-AS1, and MCM3AP-AS1 expressed at a high level in breast cancer cells. Besides, MCM3AP-AS1 was confirmed as a cytoplasmic RNA. In addition, there was a positive expression correlation between MCM3AP-AS1 and CENPF. Therefore, MCM3AP-AS1 was confirmed to regulate CENPF via competitively binding to miR-28-5p. At last, rescue assays demonstrated that knockdown of CENPF restored miR-28-5p repression-induced cellular processes in MCM3AP-AS1-silenced cells. In vivo assay revealed that MCM3AP-AS1 could hasten tumor growth in breast cancer by targeting CENPF. All results indicated that MCM3AP-AS1/miR-28-5p/CENPF axis accelerates breast cancer progression.

乳腺癌是女性中常见的癌症之一，对女性健康构成了巨大威胁。已经证实lncRNA的异常表达是癌症的重要诱导剂。通过搜索GEO和TCGA数据库，我们发现CENPF在乳腺癌组织中被上调。通过RT-qPCR，发现CENPF在乳腺癌细胞中被上调。功能实验表明，CENPF对细胞功能具有积极作用，包括增殖，迁移和侵袭。随后，确认CENPF与miR-28-5p结合，并且其表达被miR-28-5p抑制。此外，发现miR-28-5p与MCM3AP-AS1结合，并且MCM3AP-AS1在乳腺癌细胞中高水平表达。此外，MCM3AP-AS1被确认为细胞质RNA。此外，MCM3AP-AS1与CENPF之间存在正相关。因此，证实MCM3AP-AS1通过竞争性结合miR-28-5p来调节CENPF。最后，救援分析表明，敲除CENPF可以恢复MCM3AP-AS1沉默细胞中miR-28-5p阻遏诱导的细胞过程。体内试验显示，MCM3AP-AS1可通过靶向CENPF来加速乳腺癌中的肿瘤生长。所有结果表明，MCM3AP-AS1 / miR-28-5p / CENPF轴可加速乳腺癌的进展。体内试验表明，MCM3AP-AS1可通过靶向CENPF来加速乳腺癌中的肿瘤生长。所有结果表明，MCM3AP-AS1 / miR-28-5p / CENPF轴可加速乳腺癌的进展。体内试验显示，MCM3AP-AS1可通过靶向CENPF来加速乳腺癌中的肿瘤生长。所有结果表明，MCM3AP-AS1 / miR-28-5p / CENPF轴可加速乳腺癌的进展。