Abstract Vertically transmitted Epichloe endophyte can be beneficial to host grasses. In order to achieve high seed and endophyte viability under storage, knowledge of optimal seed moisture content and germination behavior of endophyte-infected grass seed is required. In the current research project, Achnatherum inebrians seeds were adjusted to different moisture contents to investigate effects on germination and viability of the endophyte, E. gansuensis, after six months of storage. To explore mechanism of the effect of moisture content and endophyte on seed germination, a number of physiological and biochemical indicators were determined, including electrical conductivity (EC), contents of proline (Pro), malondialdehyde (MDA) and total soluble sugar; activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and hydrogen peroxide (H2O2). The results showed that the viability of the endophyte at 5.5% and 9.5% seed moisture was significantly (p

中文翻译：

---

种子含水量和内生菌对蝽螟萌发和生理的影响

摘要 垂直传播的 Epichloe 内生菌对寄主草有益。为了在储存条件下实现种子和内生菌的高生存力，需要了解内生菌感染的草种子的最佳种子含水量和发芽行为。在当前的研究项目中，将 Achnatherum inebrians 种子调整为不同的水分含量，以研究储存 6 个月后对内生菌 E. gansuensis 的萌发和活力的影响。为探究水分和内生菌对种子萌发的影响机制，测定了电导率（EC）、脯氨酸（Pro）、丙二醛（MDA）、总可溶性糖等多项生理生化指标；超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶 (CAT) 和过氧化氢 (H2O2)。结果表明，内生菌在 5.5% 和 9.5% 种子水分条件下的生存力显着（p