Abstract Low temperature is one of the stresses which threaten eggplant during its whole growth period, resulting in the inhibition of growth and development and the loss of yield. It is essential to realize the key genes and pathways in response to low temperature for eggplant breeding. To compare the transcriptomics profiling of two eggplant varieties with differential low temperature tolerance, we conducted the present experiment. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were used for comparing the transcriptomics profile. Here, a total of 2854 and 2697 differentially expressed genes were identified in A153 which was selected from the local variety and locally named ‘Chengdumiqie’ and A80 (CGN22911) following the low temperature treatment. Through GO analysis, we found that sequence-specific DNA binding was the representative of GO term in the unique differentially expressed genes (DEGs) of both varieties. The KEGG analysis of unique DEGs showed that in both varieties plant hormone signal transduction was the most dominant group. Further analysis revealed that several significant differences existed in the plant hormone signal transduction between two varieties. Finally, the analysis of transcription factor families revealed the complex transcriptional regulation in both cultivars. These findings provided a new insight into the molecular mechanisms responsible for response to low temperature and genetic improvement in eggplant.

中文翻译：

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转录组学分析揭示了敏感和耐受茄子对低温的反应

摘要 低温是威胁茄子整个生育期的逆境之一，导致生长发育受到抑制，产量下降。掌握响应低温的关键基因和途径对于茄子育种至关重要。为了比较具有不同低温耐受性的两个茄子品种的转录组学分析，我们进行了本实验。基因本体论 (GO) 富集和京都基因和基因组百科全书 (KEGG) 分析用于比较转录组学特征。在这里，从当地品种中选择的A153和A80（CGN22911）经过低温处理后，共鉴定出2854和2697个差异表达基因。通过GO分析，我们发现序列特异性 DNA 结合是两个品种独特差异表达基因 (DEG) 中 GO 术语的代表。独特的DEGs的KEGG分析表明，在这两个品种中，植物激素信号转导是最主要的群体。进一步的分析表明，两个品种之间的植物激素信号转导存在一些显着差异。最后，转录因子家族的分析揭示了两个品种中复杂的转录调控。这些发现为茄子对低温的反应和遗传改良的分子机制提供了新的见解。独特的DEGs的KEGG分析表明，在这两个品种中，植物激素信号转导是最主要的群体。进一步的分析表明，两个品种之间的植物激素信号转导存在一些显着差异。最后，转录因子家族的分析揭示了两个品种中复杂的转录调控。这些发现为茄子对低温的反应和遗传改良的分子机制提供了新的见解。独特的DEGs的KEGG分析表明，在这两个品种中，植物激素信号转导是最主要的群体。进一步的分析表明，两个品种之间的植物激素信号转导存在一些显着差异。最后，转录因子家族的分析揭示了两个品种中复杂的转录调控。这些发现为茄子对低温的反应和遗传改良的分子机制提供了新的见解。