Abstract Fusarium wilt is a serious soil-borne disease for cucumber production; however, intercropping with celery and using resistant cultivars could alleviate this problem. The aims of the study were to verify whether cucumber Fusarium wilt resistance induced by intercropping with celery differs from that induced by the cucumber genotype and whether celery synthesizes organosulfur allelochemicals to inhibit the growth of Fusarium oxysporum f. sp. cucumerinum (Foc). First, biochemical and transcriptome analyses were performed for the roots of two cucumber cultivars during the response to Foc infection in a monoculture system and cucumber/celery intercropping system, and the results indicated that the Fusarium wilt-resistant cucumber cultivar and cucumber intercropped with celery resulted in different immune responses against Foc inoculation. Then, the possible allelochemicals in the fresh root and rhizosphere soil of celery were extracted with acetone, separated and purified by chromatography four times and cocultured with Foc in vitro. Three organosulfur compounds, namely, thioglycolic acid, propanethiol and isopropyl isothiocyanate, were identified from the chromatography fractions, with the highest inhibition on Foc in the fourth chromatography by gas chromatography/mass spectrometry. All these compounds exhibited inhibitory effects on Foc, but thioglycolic acid showed stronger inhibition than the other two compounds and completely inhibited the growth of Foc colonies at concentrations of 50–100 μmol L−1. The information obtained will permit new management practices that prevent and control cucumber Fusarium wilt.

中文翻译：

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芹菜间作黄瓜枯萎病抗性与黄瓜基因型不同，与含硫化感物质有关

摘要 枯萎病是黄瓜生产中一种严重的土传病害；然而，与芹菜间作和使用抗病品种可以缓解这个问题。本研究的目的是验证与芹菜间作诱导的黄瓜枯萎病抗性是否与黄瓜基因型诱导的不同，以及芹菜是否合成有机硫化感物质来抑制尖孢镰刀菌的生长。sp. 黄瓜 (Foc)。首先，在单一栽培系统和黄瓜/芹菜间作系统中对两种黄瓜品种在 Foc 感染响应期间进行了生化和转录组分析，结果表明抗枯萎病黄瓜品种和黄瓜与芹菜间作导致在针对 Foc 接种的不同免疫反应中。然后，用丙酮提取芹菜鲜根和根际土壤中可能存在的化感物质，四次层析分离纯化，与Foc体外共培养。从色谱级分中鉴定出三种有机硫化合物，即巯基乙酸、丙硫醇和异硫氰酸异丙酯，通过气相色谱/质谱法在第四次色谱中对 Foc 的抑制最高。所有这些化合物都对 Foc 显示出抑制作用，但巯基乙酸比其他两种化合物显示出更强的抑制作用，并且在 50-100 μmol L−1 的浓度下完全抑制 Foc 集落的生长。获得的信息将允许采取新的管理措施来预防和控制黄瓜枯萎病。