The current study aimed to determine how Clostridium butyricum (C. butyricum) relieved diarrhea by evaluating its effects on intestinal digestive enzyme activity, barrier integrity, inflammatory responses, and oxidative stress in weaned piglets. A total of 360 weaned piglets that were 25 days old (180 males and 180 females) were randomly assigned to 5 groups in 6 pens, with 12 pigs per pen, and offered the same antibiotic-free corn-soybean-type basal diet for 30 d. The groups were as follows: no additives (control), basal diet + 250 mg/kg C. butyricum (CB1), basal diet + 500 mg/kg C. butyricum (CB2), basal diet + 1,000 mg/kg C. butyricum (CB3), and basal diet + 2,000 mg/kg C. butyricum (CB4). The incidence of diarrhea decreased quadratically as the amount of C. butyricum supplementation was increased, and this decrease was minimized in the CB2 group. Compared with those in the control group, the duodenal amylase and lipase activities were increased quadratically and maximized in the CB2 group, and the jejunal lipase activity was increased cubically in the CB1 group, while the pancreatic trypsin activity was increased linearly and maximized in the CB4 group. The serum concentration of D-lactic acid responded to the increases in the levels of C. butyricum in a quadratic manner; the minimum level was observed in the CB2 group. Jejunal zonula occludens-1 expression displayed linear and quadratic trends following C. butyricum supplementation, with the maximum response observed in the CB3 group. Quadratic and cubic increases were observed in jejunal alkaline phosphatase activity in the CB3 group. The jejunal IL-10 concentration increased, while the jejunal vascular endothelial growth factor concentration decreased quadratically in response to dietary C. butyricum supplementation in the CB2 group. No significant differences among the groups were observed in terms of the jejunal mucosal concentrations of oxidation-associated enzymes and products. In conclusion, dietary supplementation with appropriate doses of C. butyricum could relieve diarrhea in weaned piglets by enhancing digestive enzyme activity, maintaining intestinal barrier integrity, and relieving intestinal inflammation.

当前的研究旨在通过评估其对断奶仔猪肠道消化酶活性，屏障完整性，炎症反应和氧化应激的影响来确定丁酸梭菌（C.butyricum）如何减轻腹泻。将总共​​360只25天大的断奶仔猪（雄性180只，雌性180只）随机分为5组，每组6头，每头12头，并提供相同的无抗生素玉米-大豆型基础日粮30头d。这些组如下：无添加剂（对照），基础饮食+ 250 mg / kg酪酸酪（（CB1），基础饮食+ 500 mg / kg丁酸酪氨酸（CB2），基础饮食+ 1,000 mg / kg丁酸酪。（CB3）和基础饮食+ 2,000 mg / kg丁酸梭菌（CB4）。腹泻的发生率随着丁酸梭菌补充量的增加而呈二次下降趋势，而在CB2组中这种下降最小。与对照组相比，CB2组的十二指肠淀粉酶和脂肪酶活性呈二次方增加，最大，而CB1组的空肠脂肪酶活性则呈立方增加，而胰蛋白酶的活性呈线性增加，CB4组最大。组。D-乳酸的血清浓度对丁酸梭菌水平的增加呈二次响应；在CB2组中观察到最低水平。空肠小肠闭塞1表达在丁酸梭菌之后显示线性和二次趋势补充，在CB3组中观察到最大的反应。CB3组的空肠碱性磷酸酶活性观察到二次方和三次方增加。在CB2组中，由于饮食中添加丁酸梭状芽孢杆菌，空肠IL-10浓度增加，而空肠血管内皮生长因子浓度平方降低。在空肠粘膜中与氧化相关的酶和产物的浓度方面，在各组之间没有观察到显着差异。总之，饮食中补充适当剂量的丁酸梭菌可以通过增强消化酶的活性，维持肠屏障的完整性和减轻肠道炎症来减轻断奶仔猪的腹泻。