Accurate identification of variety origin of Panax ginseng is quite necessary not only for variety conservation but also for the quality control of ginseng intermediate and terminal products. However, DNA degradation during the manufacturing processes and the low intra-specific polymorphism within P. ginseng lead to botanical origin authentication of industrial ginseng products at the variety level quite difficult. In this study, a multiple allele-specific PCR system was established based on two ‘Huangguo’ variety-specific SNPs that were respectively exploited from chloroplast ccsA and rpoC2 genes. The multiple allele-specific PCR method produced DNA amplicons of longer than 200 bp from seriously degraded samples and could detect 1 % of intentional adulteration of ‘Hongguo’ variety down to 0.01 ng level of genomic DNA. Furthermore, the developed real time allele-specific PCR enabled high-throughput genotyping of ‘Huangguo’ from local ginseng populations. This study provided a uniform method for variety origin authenticity control through the P. ginseng industrial chain and therefore can be used to maintain the high quality of industrial P. ginseng products.

准确鉴定人参的品种来源不仅对于品种保护，而且对于人参中间产品和终端产品的质量控制都是非常必要的。然而，在制造过程中的DNA降解和人参内的低种内多态性导致很难在品种级别对工业人参产品进行植物来源鉴定。在这项研究中，基于分别从叶绿体ccsA和rpoC2中利用的两个“黄果”变种特异性SNP，建立了多个等位基因特异性PCR系统。基因。多重等位基因特异性PCR方法从严重降解的样品中产生了200 bp以上的DNA扩增子，可检测到1％的“红果”变种的故意掺假，降低至0.01 ng水平的基因组DNA。此外，已开发的实时等位基因特异性PCR能够对当地人参群体的“黄果”进行高通量基因分型。这项研究为通过人参产业链进行品种起源真实性控制提供了统一的方法，因此可用于维持工业人参产品的高质量。