The aim of this study was to examine the expression of the cytokines and chemokines receptor-3 (CCR3) molecule in endothelial cells and vascular structures in a murine model of corneal neovascularization and in samples of neovascularized human corneas. An immunofluorescence assay using the murine model showed a greater proportion and intensity of CCR3 in the epithelium and corneal subepithelial regions in corneas with neovascularization. In the absence of vascularization, no CCR3 was found. Of the 32 studied tissues, eight were vascularized and 24 were avascular. In the human corneas, vascularized corneas showed positive labeling for CD31 in all the analzedtissues, as well as positive labeling for CCR3. Therefore, all vascularized tissues showed positive coexpression of CCR3 and CD31, whereas none of the avascular corneas showed immunolabeling for either of these receptors. These results suggest that CCR3 could be a possible marker for corneal neovascularization with potential to be a therapeutic target.

这项研究的目的是检查在角膜新生血管模型和新生血管化人类角膜样本中内皮细胞和血管结构中细胞因子和趋化因子受体3（CCR3）分子的表达。使用鼠模型的免疫荧光分析显示，在新血管形成的情况下，角膜上皮和角膜上皮下区域中CCR3的比例和强度更高。在没有血管形成的情况下，未发现CCR3。在研究的32个组织中，有8个血管化，有24个血管无血管。在人角膜中，血管化角膜在所有肛门组织中均显示CD31阳性标记，以及CCR3阳性标记。因此，所有血管化组织均显示CCR3和CD31阳性共表达，而无血管的角膜均未显示出针对这些受体中任何一种的免疫标记。这些结果表明，CCR3可能是角膜新血管形成的可能标志物，有可能成为治疗靶标。