Diagnostic Microbiology and Infectious Disease ( IF 2.1 ) Pub Date : 2020-05-30 , DOI: 10.1016/j.diagmicrobio.2020.115097 Avi Peretz 1 , Bat-Sheva Zadok 2 , Maya Azrad 3
Objectives
To evaluate the performance of Influ A + B K-SeT®, a rapid influenza antigen detection kit, in order to determine whether its implementation as a point-of-care test in the emergency room (ER) is justified.
Methods
Nasopharyngeal swabs (n = 170) were collected in triplicates from patients with suspected influenza infection. One sample was tested by the Influ A + B K-SeT® kit and the other two samples were analyzed with Simplexa™ Flu A/B & RSV Direct Kit and Xpert® Flu, two RT-PCR-based assays routinely used in the ER.
Results
The sensitivity and specificity of the Influ A + B K-SeT® kit were 91.2%–94.5% and 98.2%, respectively, compared to the Xpert® Flu assay and the Simplexa™ Flu A/B & RSV Direct Kit, respectively.
Conclusions
Considering the satisfactory diagnostic performance of the Influ A + B K-SeT® test, its implementation as a point-of-care is expected to aid in decision-making, minimize time-to-result and reduce healthcare costs.
中文翻译:
与两种用于检测流感病毒的RT-PCR分析相比,Influ a + BK-SeT®分析的性能。
目标
为了评估Influ A + BK-SeT®(一种快速流感抗原检测试剂盒)的性能,以确定在急诊室(ER)中将其作为即时护理测试的实施是否合理。
方法
从怀疑有流感感染的患者中一式三份收集鼻咽拭子(n = 170)。其中一个样品通过Influ A + BK-SeT®试剂盒进行测试,另外两个样品通过Simplexa™Flu A / B和RSV Direct试剂盒以及Xpert®Flu(ER中常规使用的两种基于RT-PCR的检测方法)进行分析。
结果
与Xpert®Flu检测试剂盒和Simplexa™Flu A / B和RSV Direct试剂盒相比,Influ A + BK-SeT®试剂盒的敏感性和特异性分别为91.2%–94.5%和98.2%。
结论
考虑到Influ A + BK-SeT®测试的令人满意的诊断性能,预期将其作为即时护理实施有助于决策,最小化结果时间并降低医疗保健成本。