Objectives

To evaluate the performance of Influ A + B K-SeT®, a rapid influenza antigen detection kit, in order to determine whether its implementation as a point-of-care test in the emergency room (ER) is justified.

Methods

Nasopharyngeal swabs (n = 170) were collected in triplicates from patients with suspected influenza infection. One sample was tested by the Influ A + B K-SeT® kit and the other two samples were analyzed with Simplexa™ Flu A/B & RSV Direct Kit and Xpert® Flu, two RT-PCR-based assays routinely used in the ER.

Results

The sensitivity and specificity of the Influ A + B K-SeT® kit were 91.2%–94.5% and 98.2%, respectively, compared to the Xpert® Flu assay and the Simplexa™ Flu A/B & RSV Direct Kit, respectively.

Conclusions

Considering the satisfactory diagnostic performance of the Influ A + B K-SeT® test, its implementation as a point-of-care is expected to aid in decision-making, minimize time-to-result and reduce healthcare costs.

中文翻译：

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与两种用于检测流感病毒的RT-PCR分析相比，Influ a + BK-SeT®分析的性能。

目标

为了评估Influ A + BK-SeT®（一种快速流感抗原检测试剂盒）的性能，以确定在急诊室（ER）中将其作为即时护理测试的实施是否合理。

方法

 从怀疑有流感感染的患者中一式三份收集鼻咽拭子（n = 170）。其中一个样品通过Influ A + BK-SeT®试剂盒进行测试，另外两个样品通过Simplexa™Flu A / B和RSV Direct试剂盒以及Xpert®Flu（ER中常规使用的两种基于RT-PCR的检测方法）进行分析。

结果

与Xpert®Flu检测试剂盒和Simplexa™Flu A / B和RSV Direct试剂盒相比，Influ A + BK-SeT®试剂盒的敏感性和特异性分别为91.2％–94.5％和98.2％。

结论

考虑到Influ A + BK-SeT®测试的令人满意的诊断性能，预期将其作为即时护理实施有助于决策，最小化结果时间并降低医疗保健成本。