Clostridium ljungdahlii is a representative autotrophic gas-fermenting acetogen capable of converting CO₂ and CO into biomass and multiple metabolites. The carbon fixation and conversion based on C. ljungdahlii have great potential for the sustainable production of bulk biochemicals and biofuels using industrial syngas and waste gases. With substantial recent advances in genetic manipulation tools, it has become possible to study and improve the metabolic capability of C. ljungdahlii in gas fermentation. The product scope of C. ljungdahlii has been expanded through the introduction of heterologous production pathways followed by the modification of native metabolic networks. In addition, progress has been made in understanding the physiological and metabolic mechanisms of this anaerobe, contributing to strain designs for expected phenotypes. In this review, we highlight the latest research progresses regarding C. ljungdahlii and discuss the next steps to comprehensively understand and engineer this bacterium for an improved bacterial gas bioconversion platform.

杨氏梭菌是一种代表性的自养气体发酵产乙酸菌，能够将 CO ₂和 CO 转化为生物质和多种代谢物。基于C. ljungdahlii的碳固定和转化对于使用工业合成气和废气可持续生产大宗生物化学品和生物燃料具有巨大潜力。随着基因操作工具的重大最新进展，研究和提高杨氏梭菌在气体发酵中的代谢能力已成为可能。C. ljungdahlii的产品范围已经通过引入异源生产途径以及随后对天然代谢网络的修改进行了扩展。此外，在理解这种厌氧菌的生理和代谢机制方面也取得了进展，有助于针对预期表型的菌株设计。在这篇综述中，我们重点介绍了有关C. ljungdahlii的最新研究进展，并讨论了全面了解和改造这种细菌以改进细菌气体生物转化平台的下一步。