Abstract

The fission yeast cell is shaped as a very regular cylinder ending by hemi-spheres at both cell ends. Its conserved phenotypes are often used as read-outs for classifying interacting genes and protein networks. Using Pascal and Young-Laplace laws, we proposed a framework where scaling arguments predicted shapes. Here we probed quantitatively one of these relations which predicts that the division site would be located closer to the cell end with the larger radius of curvature. By combining genetics and quantitative imaging, we tested experimentally whether altered shapes of cell end correlate with a displaced division site, leading to asymmetric cell division. Our results show that the division site position depends on the radii of curvatures of both ends. This new geometrical mechanism for the proper division plane positioning could be essential to achieve even partitioning of cellular material at each cell division.

Graphical abstract

中文翻译：

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末端和中间：裂变酵母中的整体力平衡和隔膜位置。

摘要

裂变酵母细胞的形状非常规则，呈圆柱状，两端均由半球包围。它的保守表型常被用作对相互作用基因和蛋白质网络进行分类的读数。使用Pascal和Young-Laplace定律，我们提出了一个框架，其中缩放参数可预测形状。在这里，我们定量地研究了这些关系之一，该关系预测分裂位点将以较大的曲率半径位于更靠近细胞末端的位置。通过结合遗传学和定量成像，我们实验测试了细胞末端形状的改变是否与移位的分裂位点相关，从而导致细胞分裂不对称。我们的结果表明，分割部位的位置取决于两端曲率半径。

图形概要