The high lignin content of Rosa roxburghii fruits imparts a strong rough taste, which has restricted its use in industry. Little is known about the molecular mechanisms underlying lignin biosynthesis in R. roxburghii fruits. Illumina and single-molecule real-time (SMRT) sequencing platforms were combined to analyze the transcriptomic changes during the ripening of R. roxburghii fruits. After assembly, 67,739 transcripts with an average length of 1866 bp were obtained, of which 17,470 transcripts were differentially expressed during fruit ripening. Biological pathway analysis putatively identified 25 differentially expressed genes were involved in lignin biosynthesis. Furthermore, 43 putative transcription factors were identified, including bHLH, bZip, Dof, MYB, NAC, WRKY, and zf-HD, which were previously reported to control lignin biosynthesis. Among which, 28 were positive transcription factors and 15 were negative. Quantitative RT-PCR analysis of candidate genes and transcription factors correlated with the transcriptome data. The transcriptomic data generated in this study provide a basis for further studies of fruit ripening in R. roxburghii.

中文翻译：

---

转录组分析揭示了蔷薇果实成熟期间的候选木质素相关基因和转录因子

刺梨果实木质素含量高，口感粗糙，限制了其在工业上的应用。对 R. roxburghii 果实中木质素生物合成的分子机制知之甚少。Illumina 和单分子实时 (SMRT) 测序平台相结合，分析了刺梨果实成熟过程中的转录组变化。组装后获得67,739个转录本，平均长度为1866 bp，其中17,470个转录本在果实成熟期差异表达。生物途径分析推定确定了 25 个差异表达基因参与木质素生物合成。此外，还鉴定了 43 个推定的转录因子，包括 bHLH、bZip、Dof、MYB、NAC、WRKY 和 zf-HD，这些因子之前被报道用于控制木质素生物合成。其中，28个为阳性转录因子，15个为阴性。候选基因和转录因子的定量 RT-PCR 分析与转录组数据相关。本研究中产生的转录组学数据为进一步研究刺梨果实成熟提供了基础。