The present study reports on the potential of greener Biocomposite designed by living cells of mutated Bacillus sp. integrated passively with alginate to decontaminate Nickel(II) from aqueous medium by continuous column process in order to ascertain its actual environmental application. The impact of working parameters was investigated, showing better execution at a low flow rate (4 mL min⁻¹) and highest bed depth (25 cm). The percentage removal was found to be 80.76% at saturation with a sorption capacity of ~ 54 mg g⁻¹ using an influent concentration of 50 mg L⁻¹. The dynamic behaviour of the processes was well defined by Yoon–Nelson and Thomas kinetic model. Special emphasis was directed to mass transfer phenomenon using suitable mathematical expressions while different kinetic models have their limitations. The resistance of mass transfer initially depended on porous diffusion (PD) prior to the proportion of outflow being 3%, ~ 8% and 12% and subsequent metal removal were controlled by both film mass transfer and PD for Nickel(II) solution 100, 50 and 20 mg L⁻¹, respectively. Biocomposite was characterized by FTIR, SEM-EDXA and elemental analyzer which conveniently ascertained the interactions of metal to composite through electrostatic, complexation and/or chelation. Nickel(II) was recovered to the extent of 92% using 0.1 M HCl as eluant in six consecutive cycles. The Biocomposite was also efficient to decontaminate Nickel(II) from simulated effluent, where breakthrough modeling and mass transfer kinetics revealed a similar trend to the monometallic solution, thereby ensuring its prospect for industrial practice.

本研究报告了由突变芽孢杆菌属的活细胞设计的绿色生物复合材料的潜力。通过连续柱工艺与藻酸盐被动集成以去除水性介质中的镍（II），从而确定其实际的环境应用。研究了工作参数的影响，显示了在低流速（4 mL min ^-1）和最高床层深度（25 cm）下更好的执行。脱除率被发现是80.76％的饱和度具有〜54毫克g的吸附能力^-1，使用50毫克的L的进水浓度^-1。该过程的动力学行为由Yoon–Nelson和Thomas动力学模型很好地定义。使用适当的数学表达式特别强调了传质现象，而不同的动力学模型有其局限性。传质的阻力最初取决于多孔扩散（PD），然后流出的比例分别为3％，〜8％和12％，随后的金属去除都通过薄膜传质和镍（II）溶液100的PD来控制， 50和20 mg L ^-1， 分别。FTIR，SEM-EDXA和元素分析仪对生物复合材料进行了表征，可以通过静电，络合和/或螯合方便地确定金属与复合材料的相互作用。在六个连续的循环中，使用0.1 M HCl作为洗脱液将镍（II）回收到92％的程度。该生物复合材料还可以有效地去除模拟废水中的镍（II），突破性的建模和传质动力学揭示了与单金属溶液相似的趋势，从而确保了其工业应用前景。