To investigate differences in the metabolome between ancient and modern Triticum varieties, a total of 15 varieties each of bread and durum wheat, spelt, emmer, and einkorn were cultivated and harvested at four different locations (Seligenstadt, Hohenheim, Eckartsweier, and Oberer Lindenhof), and their acetonitrile extracts were analyzed by means of UPLC–TOF MS^e. Nine marker metabolites were clearly identified using reference or surrogate standard compounds including di- and triacylglycerides as well as galactolipids and enabling the discrimination of wheat species and their growing location.

中文翻译：

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非目标UPLC–ESI–TOF-MS分析对小麦品种进行分析

为了研究古代和现代小麦品种之间代谢组间的差异，在四个不同的地点（塞利根施塔特，霍恩海姆，埃卡特斯韦尔和奥伯勒·林登霍夫）共种植和收获了面包和硬粒小麦，斯佩尔特，艾默和埃肯恩各15个变种。 ，并通过UPLC-TOF MS ^e对乙腈提取物进行了分析。使用参考或替代标准化合物（包括二酰基和三酰基甘油酯以及半乳糖脂）可以清楚地识别出九种标记代谢物，从而能够区分小麦种类及其生长位置。