Sorghum bicolor (L.) Moench is a crop that has high potential to be used for bioenergy generation. The objective of this study was to introgression of the bmr6 allele in elite lines of biomass sorghum and to obtain experimental “brown midrib” hybrids. Three genetic materials belonging to the Embrapa Maize and Sorghum Breeding Program were used. Two backcross programs were conducted separately, in which the CMSXS170 line was the donor of the bmr6 allele and the CMSXS652 and IS23777 lines were the recurrent ones. Through molecular markers specific for the bmr6 allele, the assisted selection was utilized for the brown midrib characteristic in the BC1F1 and BC2F1 generations. Polymorphic SNP markers were distributed throughout the genome of sorghum to accelerate the recovery of the recurrent genome. After the confirmation of the bmr genotypes, the lines were crossed with line A (female), to obtain the hybrid seeds and evaluated under field conditions. As result, it was possible to perform the introgression of the bmr6 allele in lines of biomass sorghum. The SNPs markers were efficient in identifying individuals with a higher rate of recurrence of the recurrent genome. Experimental “brown midrib” hybrids were obtained and demonstrated satisfactory potential for bioenergy production.

中文翻译：

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用于生物能源生产的生物质高粱系中 bmr6 等位基因的基因渗入

双色高粱 (L.) Moench 是一种具有用于生物能源生产的巨大潜力的作物。本研究的目的是将 bmr6 等位基因渗入生物质高粱的优良品系，并获得实验性的“棕色中脉”杂种。使用了属于 Embrapa 玉米和高粱育种计划的三种遗传材料。分别进行了两个回交程序，其中 CMSXS170 品系是 bmr6 等位基因的供体，CMSXS652 和 IS23777 品系是循环的。通过特定于 bmr6 等位基因的分子标记，辅助选择被用于 BC1F1 和 BC2F1 代的棕色中脉特征。多态性 SNP 标记分布在整个高粱基因组中，以加速循环基因组的恢复。bmr基因型确认后，将品系与品系A（雌性）杂交，获得杂交种子并在田间条件下进行评价。结果，可以在生物质高粱品系中进行 bmr6 等位基因的基因渗入。SNP 标记可有效识别复发基因组复发率较高的个体。获得了实验性的“棕色中脉”杂种，并显示出令人满意的生物能源生产潜力。