The study was designed to evaluate the production of auxin by eukaryotic unicellular organism Pichia fermentans. Different media formulations were used for the production of indole-3-acetic acid (IAA) under broth and submerged conditions. Wheat straw-based production medium was formulated and optimized using statistical approach. The IAA production was significantly enhanced by nine folds, when the wheat straw was pretreated with Phanerochaete chrysosporium (150 µg/ml) as compared to untreated wheat straw (16.44 µg/ml). Partial purification of IAA was carried out by silica gel column chromatography and further confirmed by high-performance liquid chromatography. Exogenous application of crude and partially purified IAA positively influenced the Vigna radiata seedling growth. The number of lateral roots in the growing seedlings was significantly higher as compared to the control seeds. Thus, the present findings point towards an efficient production of plant hormone by yeast and white rot fungus using abundantly available wheat straw, which may lead to the development of cost-effective production of such metabolites and their further use in agricultural field to reduce the negative impact of chemical fertilizers.

中文翻译：

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淹没条件下发酵发酵毕赤酵母对木质纤维素生物质进行真菌预处理以生产植物激素

该研究旨在评估真核单细胞发酵毕赤酵母的生长素产生。在肉汤和浸没条件下，将不同的培养基配方用于生产吲哚-3-乙酸（IAA）。使用统计方法配制和优化了基于小麦秸秆的生产培养基。与未处理的小麦秸秆（16.44 µg / ml）相比，用Phanerochaete chrysosporium（150μg/ ml）预处理小麦秸秆时，IAA产量显着提高了九倍。IAA的部分纯化通过硅胶柱色谱法进行，并通过高效液相色谱法进一步确认。粗制和部分纯化的IAA的外源施用对Vigna radiata产生了积极影响幼苗生长。与对照种子相比，正在生长的幼苗中侧根的数量明显更高。因此，目前的发现表明，利用大量可利用的小麦秸秆，通过酵母和白腐真菌可有效生产植物激素，这可能导致开发出具有成本效益的此类代谢产物，并将其进一步用于农业领域以减少负面影响。化肥的影响。