The quantitative studies of cell proliferation and migration under different chemical environments are important for both scientists and clinicians searching for new therapeutics. In this study, we developed a new device to pattern several types of cells in 24-well-plate and demonstrated its’ application in cancer cell proliferation and migration assay. The new device combined 3D-printed-silica-part for multi cell types loading with PDMS-through-hole-layer-part for cell micro-patterning which was matched with commercial 24-well-plate. This 24-well-plate based device is flexible and feasible in many applications and can be used in one piece or multi pieces. Besides the application for two types of cells proliferation and migration assay in one chemical condition, as a demonstration, the migration behaviors of four types of cells under 24 types of EGF + bFGF combinatorial conditions were studied. We believed this device could be widely used in clinical searching for new anti-cancer therapeutics and other related studies.

中文翻译：

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通过基于24孔板的新型设备在组合化学环境下进行高通量细胞迁移分析。

在不同化学环境下对细胞增殖和迁移的定量研究对于寻找新疗法的科学家和临床医生都非常重要。在这项研究中，我们开发了一种可在24孔板中对几种类型的细胞进行模式化的新设备，并证明了其在癌细胞增殖和迁移测定中的应用。新设备结合了用于多种细胞类型装载的3D打印硅胶部分和用于细胞微图案化的PDMS通孔层部分，并与商用24孔板相匹配。这种基于24孔板的设备在许多应用中都灵活且可行，并且可以一件或多件使用。除了在一种化学条件下适用于两种类型的细胞增殖和迁移测定的应用之外，例如，研究了四种类型的细胞在24种EGF + bFGF组合条件下的迁移行为。我们认为该设备可广泛用于临床寻找新的抗癌疗法和其他相关研究。