One promising strategy to engineer plants that are resistant to plant pathogens involves transforming plants with RNA silencing constructs for resistance to multiple pathogens. Garden bean is significantly damaged by bean common mosaic virus (BCMV), bean common mosaic necrosis virus (BCMNV) and cucumber mosaic virus (CMV). In this study, we prepared constructs producing sense, antisense and hairpin RNA (hpRNA) structures to target single as well as multiple viruses. Silencing efficiency of these constructions was analyzed using Agrobacterium (GV3101) transient expression in Nicothinia bethamiana and Phaseolus vulgaris plants. The results showed significantly reduced disease symptoms and virus accumulation in N. bethamiana plants. Generally, the efficiency of the prepared constructs was hairpin, antisense and sense, respectively, and also, there was a significant difference between mono-gene and multiple-gene constructs for reducng virus accumulation and the multiple-gene constructs showed higher effectiveness. Experiments in this study showed that using Agrobacterium harboring binary constructs containing a Caenorhabditis elegans gene, Ced-9, or a plant gene, AtBag-4, anti-apoptosis gene as a mix suspension with an Agrobacterium containing pFGC-BNC.h, a plasmid containing multiple gene fragments consisting of BCMV-CP, BCMNV-HC-Pro and CMV-2b, improved the efficiency of pFGC-BNC.h transformation. We showed reduced virus accumulation in these transgenic bean plans.

工程改造对植物病原体具有抗性的植物的一种有前途的策略涉及用RNA沉默构建体转化对多种病原体具有抗性的植物。菜豆受到菜豆普通花叶病毒（BCMV），菜豆普通花叶坏死病毒（BCMNV）和黄瓜花叶病毒（CMV）的严重破坏。在这项研究中，我们制备了可产生有义，反义和发夹RNA（hpRNA）结构的构建体，以靶向单一或多种病毒。使用农杆菌（GV3101）在烟碱烟草和菜豆中的瞬时表达来分析这些构建物的沉默效率。结果表明，N。bethamiana的疾病症状和病毒积累明显减少植物。通常，制备的构建体的效率分别为发夹，反义和有义，并且在减少病毒积累的单基因和多基因构建体之间也存在显着差异，并且多基因构建体显示出更高的效力。这项研究的实验表明，使用农杆菌具有包含秀丽隐杆线虫基因Ced-9或植物基因At Bag-4的二元构建体，将其作为抗凋亡基因与含有p FGC-BNC.h的农杆菌混合悬液。含有由BCMV-CP，BCMNV-HC-Pro和CMV-2b组成的多个基因片段的质粒，提高了p FGC-BNC.h的效率转型。我们在这些转基因豆计划中显示出减少的病毒积累。