2-Phenylethanol (2-PE) with a pleasant rose-like odor is a valuable aroma compound used in many fields. 2-PE production by yeast is considered a promising alternative to chemical synthesis and extraction from natural materials. In this report, the strain YF1702 produced a significantly higher level of 2-PE when compared with other strains isolated from Baijiu-producing environments. According to morphological properties, physiological and biochemical characteristics, and 26S rDNA sequence analysis, strain YF1702 was identified as Pichia kudriavzevii. The optimal fermentation conditions of YF1702 for producing 2-PE were obtained by single-factor experiments, Plackett–Burman design, steepest ascent design, and response surface methodology. The optimal inoculation conditions for strain YF1702 were 50 g/L glucose, 6.0 g/L yeast extract, 10.7 g/L L-Phe, and 32 g/L Tween-60. The optimal fermentation conditions were pH 2.3, 26 °C, 210 rpm shaking, an inoculum size of 0.4% (v/v), and a loading volume of 25.5 mL/250 mL for 56 h. Under these optimal conditions 2-PE production by YF1702 was 5.09 g/L. This strain has the potential to increase the content of 2-PE in Baijiu production and enhance the aroma characteristics of Baijiu.

具有令人愉快的玫瑰般气味的2-苯基乙醇（2-PE）是一种用于许多领域的有价值的芳香化合物。酵母生产2-PE被认为是化学合成和从天然材料提取的有前途的替代方法。在该报告中，与从白酒生产环境中分离的其他菌株相比，菌株YF1702产生了更高水平的2-PE 。根据形态学特性，生理生化特性和26S rDNA序列分析，菌株YF1702被鉴定为库氏毕赤酵母。通过单因素实验，Plackett-Burman设计，最陡峭的上升设计和响应面方法，获得了用于生产2-PE的YF1702的最佳发酵条件。YF1702菌株的最佳接种条件为50 g / L葡萄糖，6.0 g / L酵母提取物，10.7 g / L L-Phe和32 g / L Tween-60。最佳发酵条件为pH 2.3、26°C，210 rpm摇动，接种量为0.4％（v / v），56 h的装载量为25.5 mL / 250 mL。在这些最佳条件下，YF1702生产的2-PE为5.09 g / L。该菌株具有提高2-PE在内容的潜力白酒生产和增强的特征香气喝白酒。