Rapid, ultrasensitive and highly specific biosensor for the diagnosis of SARS-CoV-2 in clinical blood samples,Materials Chemistry Frontiers

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Rapid, ultrasensitive and highly specific biosensor for the diagnosis of SARS-CoV-2 in clinical blood samples
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2020-05-29 , DOI: 10.1039/d0qm00294a
Lu Zeng _{1,

2,

3,

4,

5} , Yue Li _{1,

2,

3,

4,

5} , Jie Liu _{1,

2,

3,

4,

5} , Lingling Guo _{1,

2,

3,

4,

5} , Zhongxing Wang _{1,

2,

3,

4,

5} , Xinxin Xu _{1,

2,

3,

4,

5} , Shanshan Song _{1,

2,

3,

4,

5} , Changlong Hao _{1,

2,

3,

4,

5} , Liqiang Liu _{1,

2,

3,

4,

5} , Meiguo Xin _{4,

6,

7,

8} , Chuanlai Xu _{1,

2,

3,

4,

5}

Affiliation

In this study, a lateral flow combined IgG–IgM immunochromatographic assay is developed for the rapid, simultaneous detection of IgM and IgG antibodies against SARS-CoV-2 in clinical blood samples within 15 min. The clinical detection sensitivity and specificity of the assay strips is investigated in samples of blood from inpatients with COVID-19. The sensitivity and specificity of this assay are 85.29% and 100.00%, respectively. Compared with a single IgG and IgM test, the combined IgG–IgM immunochromatographic strip test has higher sensitivity. Our results demonstrate that the combined IgG–IgM immunochromatographic strip is suitable for the rapid screening of SARS-CoV-2 infection, among confirmed COVID-19 patients, suspect patients and asymptomatic SARS-CoV-2 carriers.

中文翻译：

快速，超灵敏，高度特异性的生物传感器，用于诊断临床血样中的SARS-CoV-2

在这项研究中，开发了一种侧向流式结合的IgG–IgM免疫色谱分析方法，可在15分钟内快速，同时检测抗SARS-CoV-2的IgM和IgG抗体。在来自COVID-19住院患者的血液样本中研究了检测条的临床检测灵敏度和特异性。该测定的灵敏度和特异性分别为85.29％和100.00％。与单次IgG和IgM测试相比，IgG-IgM免疫层析试纸组合检测具有更高的灵敏度。我们的结果表明，在确诊的COVID-19患者，疑似患者和无症状SARS-CoV-2携带者中，IgG-IgM免疫层析组合试纸适用于SARS-CoV-2感染的快速筛查。

更新日期：2020-07-02

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