A microbe-based silver nanoparticle could inhibit the tobacco mosaic virus. In the present study, AgNO3 reacts with Pseudomonas fluorescens CZ strain fermentative broth and formed silver nanoparticles. The crystallinity and purity of silver nanoparticles (AgNPs) were examined by an X-ray diffractometer. Number of Bragg reflection was indexed in the face-centered AgNPs to show a cubic structure. Observation of scan electron microscope (SEM) confirmed the formation of AgNPs. Antiviral effects determined by percentage inhibition of local lesion of tobacco mosaic virus (TMV). Spray of AgNPs and inoculation with TMV assayed analysis revealed percentage inhibition of local lesion 92.70. Effects of AgNPs on the morphology of TMV were observed by a transmission electron microscope (TEM). Micrograph images confirmed the significant effects on the morphology of TMV. Biogenic NP from AgNO3 and fermented broth from Pseudomonas fluorescens could be a potent approach to control the TMV.

中文翻译：

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荧光假单胞菌生物制备银纳米颗粒以控制烟草花叶病毒

基于微生物的银纳米颗粒可以抑制烟草花叶病毒。在本研究中，AgNO3与荧光假单胞菌CZ菌株发酵液反应并形成银纳米颗粒。用X射线衍射仪检查银纳米颗粒（AgNPs）的结晶度和纯度。布拉格反射的数量在以面部为中心的AgNP中进行了索引，以显示立方结构。扫描电子显微镜（SEM）的观察证实了AgNP的形成。抗病毒作用通过抑制烟草花叶病毒（TMV）的局部病变百分比来确定。喷洒AgNPs并接种TMV分析后发现局部病变的抑制百分比为92.70。通过透射电子显微镜（TEM）观察到AgNPs对TMV形态的影响。显微照片证实了对TMV形态的显着影响。来自AgNO3的生物NP和来自荧光假单胞菌的发酵肉汤可能是控制TMV的有效方法。