In the poultry and livestock industries, precise genetic information is crucial for improving economic traits. Thus, functional genomic studies help to generate faster, healthier, and more efficient animal production. Chicken myoblast cells, which are required for muscle development and regeneration, are particularly important because chicken growth is closely related to muscle mass. In this study, we induced expression of microRNA-146b-5p mediated by the piggyBac transposon system in primary chicken myoblast (pCM) cells. Subsequently, we analyzed and compared the proliferation and differentiation capacity and also examined the expression of related genes in regular pCM (rpCM) cells and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells). pCM-146b OE cells showed increased proliferation and upregulated gene expression related to cell proliferation. In addition, next-generation sequencing analyses were performed to compare global gene expression patterns between rpCM cells and pCM-146b OE cells. We found that the higher proliferation in pCM-146b OE cells was the result of upregulation of gene sets related to the cell cycle. Moreover, miRNA-146b-5p overexpression had inhibitory effects on myotube differentiation in pCM cells. Collectively these results demonstrate that miR-146b-5p is closely related to the proliferation and differentiation of chicken myogenic cells as a modulator of post-transcription.

中文翻译：

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鸡成肌细胞在成肌增殖和分化过程中miRNA-146b-5p的功能分析。

在禽畜工业中，精确的遗传信息对于改善经济性状至关重要。因此，功能基因组研究有助于产生更快，更健康，更有效的动物生产。肌肉发育和再生所需的鸡成肌细胞特别重要，因为鸡的生长与肌肉质量密切相关。在这项研究中，我们诱导了piggyBac转座子系统介导的microRNA-146b-5p在原代鸡成肌细胞（pCM）中的表达。随后，我们分析并比较了它们的增殖和分化能力，还检查了相关基因在正常pCM（rpCM）细胞和过表达miRNA-146b-5p（pCM-146b OE细胞）的pCM细胞中的表达。pCM-146b OE细胞显示出增加的增殖和与细胞增殖有关的基因表达上调。此外，进行了下一代测序分析，以比较rpCM细胞和pCM-146b OE细胞之间的整体基因表达模式。我们发现pCM-146b OE细胞中较高的增殖是与细胞周期相关的基因集上调的结果。此外，miRNA-146b-5p过表达对pCM细胞的肌管分化具有抑制作用。这些结果共同证明，miR-146b-5p作为转录后调节剂与鸡肌原细胞的增殖和分化密切相关。我们发现pCM-146b OE细胞中较高的增殖是与细胞周期相关的基因集上调的结果。此外，miRNA-146b-5p过表达对pCM细胞的肌管分化具有抑制作用。这些结果共同证明，miR-146b-5p作为转录后调节剂与鸡肌原细胞的增殖和分化密切相关。我们发现pCM-146b OE细胞中较高的增殖是与细胞周期相关的基因集上调的结果。此外，miRNA-146b-5p过表达对pCM细胞的肌管分化具有抑制作用。这些结果共同证明，miR-146b-5p作为转录后调节剂与鸡肌原细胞的增殖和分化密切相关。