Recently, we demonstrated that the consumption of a bolus of bilberry extract modulates the transcription of Nrf2-regulated genes in peripheral blood lymphocytes (PBL) of healthy volunteers, accompanied by decreased DNA-damage. In the present study, we addressed the question whether consumption of consumer-relevant amounts of anthocyanin-rich beverages can achieve similar effects. The impact of three different anthocyanin-rich beverages on Nrf2-dependent gene transcription as well as and the status of DNA-damage in whole blood was investigated. After a polyphenol-reduced diet, five healthy male subjects consumed a bolus (700 mL) of respective test beverages with blood sampling up to 8 h after intake. All beverages affected the transcription of Nrf2, HO-1 and NQO-1, but showed different potencies and persistence of effects. Consumption of red fruit juice significantly reduced total DNA strand breaks (with formamidopyrimidine-DNA-glycosylase-(fpg) treatment) after 8 h in blood samples of the volunteers, suggesting antioxidant and DNA protective effects, albeit transcript levels of Nrf2-dependent genes had reached the basal state. The amount of basic DNA strand breaks (damage without oxidative DNA strand breaks) remained unchanged during the monitoring period. In contrast, a beverage prepared from grape skin extract significantly increased basic and total DNA strand breaks 2 h after intake, underlining the necessity of further investigations regarding composition, safety and consumer´s acceptance of respective products to exclude undesired adverse effects. Consumption of a bolus of anthocyanin-rich beverages affected Nrf2 and Nrf2-dependent gene transcription in human PBL and DNA integrity, which is indicative for systemic effects.

中文翻译：

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饮用富含花青素的饮料会影响健康志愿者外周淋巴细胞中的 Nrf2 和 Nrf2 依赖性基因转录以及 DNA 完整性。


最近，我们证明，食用越橘提取物可以调节健康志愿者外周血淋巴细胞 (PBL) 中 Nrf2 调节基因的转录，同时减少 DNA 损伤。在本研究中，我们解决了消费与消费者相关量的富含花青素的饮料是否可以达到类似效果的问题。研究了三种不同的富含花青素的饮料对 Nrf2 依赖性基因转录以及全血中 DNA 损伤状态的影响。在减少多酚饮食后，五名健康男性受试者饮用了各自的测试饮料，并在摄入后 8 小时内进行了血液采样。所有饮料都会影响 Nrf2、HO-1 和 NQO-1 的转录，但表现出不同的效力和效果的持久性。饮用红色果汁 8 小时后，志愿者血液样本中的总 DNA 链断裂（用甲酰胺嘧啶-DNA-糖基化酶（fpg）处理）显着减少，这表明抗氧化和 DNA 保护作用，尽管 Nrf2 依赖性基因的转录水平有所降低。达到了基础状态。在监测期间，基本DNA链断裂（无氧化DNA链断裂的损伤）的数量保持不变。相比之下，由葡萄皮提取物制备的饮料在摄入后 2 小时显着增加了基本 DNA 链断裂和总 DNA 链断裂，这强调了有必要进一步研究有关产品的成分、安全性和消费者对相应产品的接受程度，以排除不良副作用。饮用大量富含花青素的饮料会影响人类 PBL 中的 Nrf2 和 Nrf2 依赖性基因转录和 DNA 完整性，这表明存在全身效应。