Eco-Corona vs Protein Corona: Effects of Humic Substances on Corona Formation and Nanoplastic Particle Toxicity in Daphnia magna.,Environmental Science & Technology

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Eco-Corona vs Protein Corona: Effects of Humic Substances on Corona Formation and Nanoplastic Particle Toxicity in Daphnia magna.
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2020-05-28 , DOI: 10.1021/acs.est.0c00615
Oluniyi O Fadare _{1,

2,

3} , Bin Wan _{1,

2,

3} , Keyang Liu ₁ , Yu Yang ₃ , Lixia Zhao ₃ , Liang-Hong Guo ₄

Affiliation

Despite many studies on the toxicity of nanoplastic particles (NPPs) to aquatic invertebrates, the effects of ecological constituents such as humic substances (HSs) are often neglected. In our study, Daphnia magna was used to evaluate the effects of three HSs, natural organic matter (NOM), fulvic acid (FA), and humic acid (HA), on NPP toxicity and corona formation. Acute toxicities of NPPs were reduced by all HSs at environmentally relevant concentrations. NPPs elicited the upregulation of all genes related to detoxification, oxidative stress, and endocrine activity after 7 days of exposure. The presence of NOM or HA resulted in the mitigation of gene expression, whereas significantly higher upregulation of all of the genes was observed with FA. The presence of FA led to increased protein adsorption on NPPs in D. magna culture medium (eco-corona, EC) and homogenates (protein corona, PC), while there was less adsorption in the presence of HA. The highly abundant proteins identified in EC are involved in immune defense, cell maintenance, and antipredator response, while those in PC are responsible for lipid transport, antioxidant effects, and estrogen mediation. Our findings revealed the key influence of HSs on the toxicity of NPPs and provide an analytical and conceptual foundation for future study.

中文翻译：

生态电晕与蛋白质电晕：腐殖质对大型水蚤中电晕形成和纳米塑料颗粒毒性的影响。

尽管对纳米塑料颗粒（NPPs）对水生无脊椎动物的毒性进行了许多研究，但腐殖质（HSs）等生态成分的影响往往被忽视。在我们的研究中，大型蚤用于评估三种 HS，天然有机物 (NOM)、黄腐酸 (FA) 和腐植酸 (HA) 对 NPP 毒性和电晕形成的影响。所有 HS 在环境相关浓度下都降低了 NPP 的急性毒性。NPPs 在暴露 7 天后引起与解毒、氧化应激和内分泌活性相关的所有基因的上调。NOM 或 HA 的存在导致基因表达的减轻，而用 FA 观察到所有基因的显着上调。FA 的存在导致D. magna中 NPP 上的蛋白质吸附增加培养基（生态电晕，EC）和匀浆（蛋白质电晕，PC），而在 HA 存在下吸附较少。EC 中鉴定的高度丰富的蛋白质参与免疫防御、细胞维持和抗捕食者反应，而 PC 中的蛋白质负责脂质转运、抗氧化作用和雌激素介导。我们的研究结果揭示了 HSs 对 NPPs 毒性的关键影响，并为未来的研究提供了分析和概念基础。

更新日期：2020-07-07

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