The Xo1 locus in the heirloom rice variety Carolina Gold Select confers resistance to bacterial leaf streak and bacterial blight, caused by Xanthomonas oryzae pvs. oryzicola and oryzae, respectively. Resistance is triggered by pathogen-delivered transcription activator-like effectors (TALEs) independent of their ability to activate transcription, and is suppressed by variants called truncTALEs common among Asian strains. By transformation of the susceptible variety Nipponbare, we show that one of 14 nucleotide-binding, leucine-rich repeat (NLR) protein genes at the locus, with a zfBED domain, is the Xo1 gene. Analyses of published transcriptomes revealed that the Xo1-mediated response is similar to those of NLR resistance genes Pia and Rxo1 and distinct from that associated with induction of the executor resistance gene Xa23, and that a truncTALE dampens or abolishes activation of defense-associated genes by Xo1. In Nicotiana benthamiana leaves, fluorescently-tagged Xo1 protein, like TALEs and truncTALEs, localized to the nucleus. And, endogenous Xo1 specifically co-immunoprecipitated from rice leaves with a pathogen-delivered, epitope-tagged truncTALE. These observations suggest that suppression of Xo1-function by truncTALEs occurs through direct or indirect physical interaction. They further suggest that effector co-immunoprecipitation may be effective for identifying or characterizing other resistance genes.

中文翻译：

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水稻Xo1抗性基因的克隆及其与防御防御性黄单胞菌效应子Tal2h的相互作用

传家宝水稻品种Carolina Gold Select中的Xo1基因座赋予了对由黄单胞菌（Xanthomonas oryzae pvs）引起的细菌叶片条纹和枯萎病的抗性。oryzicola和oryzae。抗性由病原体传递的转录激活因子样效应物（TALE）触发，而与它们激活转录的能力无关，并被亚洲菌株中常见的称为truncTALE的变体抑制。通过易感品种日本晴的转化，我们显示了在具有zfBED结构域的14个核苷酸结合，富含亮氨酸的重复序列（NLR）蛋白基因之一是Xo1基因。对已发表的转录组的分析表明，Xo1介导的应答与NLR抗性基因Pia和Rxo1的应答相似，并且不同于与执行者抗性基因Xa23的诱导相关的应答，并且truncTALE会抑制或消除Xo1对防御相关基因的激活。在本氏烟草叶片中，荧光标记的Xo1蛋白（如TALE和truncTALEs）位于细胞核。而且，内源性Xo1与病原体传递的抗原决定簇标记的truncTALE从稻叶中特异性免疫共沉淀。这些观察结果表明，truncTALE对Xo1功能的抑制作用是通过直接或间接的物理相互作用而发生的。他们进一步表明，效应子免疫共沉淀可能有效地鉴定或表征其他抗性基因。内源Xo1与病原体传递的抗原决定簇标记的truncTALE从水稻叶片中特异性免疫共沉淀。这些观察结果表明，truncTALE对Xo1功能的抑制作用是通过直接或间接的物理相互作用而发生的。他们进一步表明，效应子免疫共沉淀可能有效地鉴定或表征其他抗性基因。内源Xo1与病原体传递的抗原决定簇标记的truncTALE从水稻叶片中特异性免疫共沉淀。这些观察结果表明，truncTALE对Xo1功能的抑制作用是通过直接或间接的物理相互作用而发生的。他们进一步表明，效应子免疫共沉淀可能有效地鉴定或表征其他抗性基因。