It remains challenging to construct a complete cell lineage map of the origin of vascular endothelial cells in any vertebrate embryo. Here, we report the application of in toto light-sheet fluorescence imaging of embryos to tracing the origin of vascular endothelial cells (ECs) at single-cell resolution in zebrafish. We adapted a known method to mount embryos and document light-sheet imaging, created an alignment, fusion, and extraction all-in-one software (AFEIO) for processing big data, and performed quantitative analysis of cell lineage relationships using Imaris software. Our data revealed that vascular ECs originated from broad regions of the gastrula along the dorsal-ventral and anterior-posterior axes, of which the dorsal-anterior cells contributed to cerebral ECs, the dorsal-lateral cells to anterior trunk ECs, and the ventral-lateral cells to posterior trunk and tail ECs. Therefore, this work, to our knowledge, charts the first comprehensive map of the gastrula origin of vascular ECs in vertebrate embryos.

中文翻译：

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浅层荧光成像绘制了斑马鱼早期胚胎中血管内皮细胞的胃胚起源

构建任何脊椎动物胚胎中血管内皮细胞起源的完整细胞谱图仍然具有挑战性。在这里，我们报告in toto的应用光斑荧光成像的胚胎，以追踪斑马鱼中单细胞分辨率的血管内皮细胞（EC）的起源。我们采用了一种已知的方法来安装胚胎和记录光片图像，创建了用于处理大数据的对齐，融合和提取多合一软件（AFEIO），并使用Imaris软件进行了细胞谱系关系的定量分析。我们的数据显示，血管内皮细胞起源于沿腹-腹和前后轴的腹肌的宽阔区域，其中背-前壁细胞促成脑部ECs，背-侧细胞促成前躯干ECs和腹侧-外侧细胞至后躯干和尾部EC。因此，据我们所知，这项工作绘制了脊椎动物胚胎中血管内皮祖细胞起源的第一个综合图。