Commercial live cell imaging systems represent a large financial burden to research groups, while current open source incubator microscopy systems lack adaptability and are sometimes inadequate for complex imaging experimentation. We present here a low-cost microscope designed for inclusion within a conventional tissue culture incubator. The build is constructed using an entry level 3D printer as the basis for the motion control system, with Raspberry Pi imaging and software integration, allowing for reflected, oblique, and fluorescence imaging of live cell monolayers. The open source nature of the design is aimed to facilitate adaptation by both the community at large and by individual researchers/groups. The development of an adaptable and easy-to-use graphic user interface (GUI) allows for the scientist to be at the core of experimental design. Simple modifications of the base GUI code, or generation of an entirely purpose-built script, will allow microscopists to place their experimental design as the priority, as opposed to designing experiments to fit their current equipment. The build can be constructed for a cost of roughly €1000 and thus serves as a low-cost and adaptable addition to the open source microscopy community.

中文翻译：

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The Incubot：基于3D打印机的显微镜，用于组织培养培养箱内的长期活细胞成像

商业活细胞成像系统给研究小组带来了沉重的财务负担，而当前的开源孵化器显微镜系统缺乏适应性，有时还不足以进行复杂的成像实验。我们在这里介绍一种低成本显微镜，该显微镜设计用于包含在常规组织培养箱中。该构建使用入门级3D打印机作为运动控制系统的基础进行构建，并具有Raspberry Pi成像和软件集成功能，可以对活细胞单层进行反射，倾斜和荧光成像。设计的开源性质旨在促进整个社区和个人研究人员/团体的适应。适应性强且易于使用的图形用户界面（GUI）的发展使科学家成为实验设计的核心。对基本GUI代码的简单修改，或生成完全专用的脚本，将使显微学家将他们的实验设计作为优先事项，而不是设计适合其当前设备的实验。该构建版本的成本约为1000欧元，因此可作为开源显微镜社区的低成本且适应性强的产品。