Biofouling on the surface of implanted medical devices severely hinders device functionality and drastically shortens device lifetime. Poly(ethylene glycol) and zwitterionic polymers are currently considered “gold standard” device coatings to reduce biofouling. To discover novel anti-biofouling materials, we created a combinatorial library of polyacrylamide-based copolymer hydrogels and screened their ability to prevent fouling from serum and platelet-rich plasma in a high-throughput parallel assay. We found certain non-intuitive copolymer compositions exhibit superior antibiofouling properties over current gold standard materials, and employed machine learning to identify key molecular features underpinning their performance. For validation, we coated the surfaces of electrochemical biosensors with our hydrogels and evaluated their anti-biofouling performance in vitro and in vivo in rodent models. Our copolymer hydrogels preserved device function and enabled continuous measurements of a small-molecule drug in vivo better than gold standard coatings. The novel methodology we describe enables the discovery of anti-biofouling materials that can extend the lifetime of real-time in vivo sensing devices.

中文翻译：

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用于防止可植入生物传感器上生物污染的组合聚丙烯酰胺水凝胶

植入式医疗设备表面的生物污垢严重阻碍了设备的功能，并大大缩短了设备的使用寿命。聚（乙二醇）和两性离子聚合物目前被认为是减少生物污垢的“黄金标准”设备涂层。为了发现新的抗生物污垢材料，我们创建了一个聚丙烯酰胺基共聚物水凝胶的组合库，并在高通量平行测定中筛选了它们防止血清和富含血小板的血浆污染的能力。我们发现某些非直观的共聚物组合物表现出优于当前金标准材料的抗生物污垢性能，并采用机器学习来识别支撑其性能的关键分子特征。为了验证，在啮齿动物模型中的体外和体内。我们的共聚物水凝胶保留了设备功能，并且能够比金标准涂层更好地在体内连续测量小分子药物。我们描述的新方法能够发现可以延长实时体内传感设备寿命的抗生物污垢材料。