Dual-Mode Detection of Bacterial 16S Ribosomal RNA in Tissues.,ACS Sensors

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Dual-Mode Detection of Bacterial 16S Ribosomal RNA in Tissues.
ACS Sensors ( IF 8.2 ) Pub Date : 2020-05-29 , DOI: 10.1021/acssensors.0c00252
Shengnan Xu ₁ , Qingyang Zhang ₁ , Xiaowan Han ₁ , Yali Wang ₁ , Xiang Wang ₁ , Marc Nazare ₂ , Jian-Dong Jiang ₁ , Hai-Yu Hu ₁

Affiliation

The specific detection of pathogens has long been recognized as a vital strategy for controlling bacterial infections. Herein, a novel hydrophilic aromatic-imide-based thermally activated delayed fluorescence (TADF) probe, AI–Cz–Neo, is designed and synthesized by the conjugation of a TADF emitter with a bacterial 16S ribosomal RNA-targeted moiety, neomycin. Biological data showed for the first time that AI–Cz–Neo could be successfully applied for the dual-mode detection of bacterial 16S rRNA using confocal fluorescence imaging and time-resolved fluorescence imaging (TRFI) in both cells and tissues. These findings greatly expand the application of TADF fluorophores in time-resolved biological imaging and provide a promising strategy for the precise and reliable diagnosis of bacterial infections based on the dual-mode imaging of bacterial 16S rRNA by fluorescence intensity and fluorescence lifetime.

中文翻译：

组织中细菌16S核糖体RNA的双模式检测。

长期以来，病原体的特异性检测一直被认为是控制细菌感染的重要策略。在本文中，一种新型的基于亲水性芳族酰亚胺的热活化延迟荧光（TADF）探针AI–Cz–Neo是通过TADF发射体与细菌16S核糖体RNA靶向部分新霉素的偶联而设计和合成的。生物学数据首次表明AI–Cz–Neo可以成功地应用于共聚焦荧光成像和时间分辨荧光成像（TRFI）在细胞和组织中细菌16S rRNA的双模式检测。这些发现大大扩展了TADF荧光团在时间分辨生物学成像中的应用，并为通过荧光强度和荧光寿命对细菌16S rRNA的双模式成像提供了一种准确而可靠的细菌感染诊断策略。

更新日期：2020-06-26

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