Multiple particle tracking microrheology (MPT) is a passive microrheological technique that measures the Brownian motion of probe particles embedded in a sample to characterize material rheological properties. MPT is a powerful tool that quantifies material rheology in the low moduli range while requiring only small sample volumes and relatively simple data acquisition using video microscopy. MPT quantitatively characterizes spatiotemporal rheological properties and is particularly well suited for the investigation of evolving materials with complex microenvironments. MPT has expanded the study of a variety of materials including biofilms, colloidal gels, hydrogels, stimuli-responsive materials, and cell-laden biomaterials. The aim of this Tutorial is to summarize the fundamentals, illustrate the versatility, and highlight recent advances in MPT. In each application, we will highlight how MPT is uniquely positioned to gather rheological properties, which would be difficult, if not impossible, to attain with other rheological characterization techniques and highlight how MPT can be used to supplement other measurement techniques. This Tutorial should provide researchers with the fundamental basis and skills needed to use MPT and develop new MPT techniques to characterize materials for their unique applications.

中文翻译：

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多粒子跟踪微流变表征：基础、新兴技术和应用

多粒子追踪微流变学 (MPT) 是一种被动微流变技术，可测量嵌入样品中的探针粒子的布朗运动，以表征材料的流变特性。MPT 是一种强大的工具，可以量化低模量范围内的材料流变，同时只需要使用视频显微镜进行少量样品和相对简单的数据采集。MPT 定量表征时空流变学特性，特别适合研究具有复杂微环境的演化材料。MPT 扩大了对各种材料的研究，包括生物膜、胶体凝胶、水凝胶、刺激响应材料和载有细胞的生物材料。本教程的目的是总结基础知识，说明多功能性，并突出 MPT 的最新进展。在每个应用中，我们将重点介绍 MPT 如何以独特的方式收集流变学特性，这对于其他流变学表征技术来说是很难甚至不可能实现的，并重点介绍 MPT 如何用于补充其他测量技术。本教程应为研究人员提供使用 MPT 和开发新的 MPT 技术来表征材料以使其独特应用所需的基本基础和技能。