Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.

中文翻译：

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粗酶漆酶生产的新方法及其在新兴污染物生物修复中的应用

白腐真菌的漆酶是一种潜在的生物催化剂，可氧化诸如农药，药物和类固醇激素等新兴污染物（EC）。这项研究旨在开发一个三步治疗EC的平台：（i）酶的产生，（ii）酶的浓度和（iii）酶的应用。第一步，比较了固体培养和液体培养。固体培养物产生的漆酶比液体培养物要多得多（八天后为447 vs. 74 µM / min），表明白腐真菌在固体培养基上繁殖。第二步，使用超滤（UF）工艺将酶浓缩6.6倍，漆酶活性为2980 µM / min。没有观察到由于过滤和膜吸附引起的酶损失，这表明超滤膜用于酶浓缩的可行性。第三步，将浓缩的粗酶应用于酶膜反应器（EMR）中，以去除多种EC（六组中的31种化合物）。EMR有效去除了类固醇激素，植物雌激素，紫外线（UV）过滤器和工业化学品（超过90％）。但是，它对农药和药物的去除率很低。