Scented (joha) and black rice indigenous to northeast region (NER) of India are the two among 40,000 varieties of species Oryza sativa, prevalent for its great aroma, medicinal property, and/or equally noteworthy taste. Biochemical and target-based liquid chromatography mass spectrometry (LCMS) analysis was performed to identify and quantify the different phytonutrients from the selected rice grains of those two varieties. Biochemical assay revealed that the selected black rice (Chakhao Amubi) contains ∼1.8-fold higher amount of total phenolic and ∼2.3-fold higher amount of total flavonoid than the scented rice grain (Kon joha). The total starch content was significantly lower in scented rice in comparison to black rice grain. The health beneficial ratio of ω-6/ω-3 essential unsaturated fatty acid is notably better in scented rice grain than black rice grain. The targeted LC-MS/MS analysis confirms the presence of oryzanol and ferulic acid in both the samples. The presence of 4-hydroxy benzoic acid, apigenin, tricin, avenasterol, coumarin, coumaric acid, phenyl alanine, caffeic acid, and α-tocophenol were confirmed in the scented rice, whereas the black rice confirms the presence of protocatechuic acid and dehydroxy myricetin. Further the quantitative analysis showed that the lipids lysophosphatidylinositol (LPI) 16:0, lysophosphatidyl ethanolamine (LPE) 14:0, lysophosphatidyl choline (LPC) 18:2, LPE 18:2, phosphatidyl etanolamine (PE), along with oryzanol, hydroxy docosanoic acid are at least threefold higher in scented rice varietal; whereas, in Chakhao Amubi, the content of petunidin galactoside, LMMPE18:2, PC14:0 are higher than the scented rice grain. In conclusion, different phytonutrients including phenol, polyphenol, and flavonoid have been identified as bioactive phytochemicals in selected rice varietals. PRACTICAL APPLICATION: This work will provide the information about the nutritional benefit of studied rice varietals. The used targeted LC-MS/MS analysis will provide the one-step information about the bioactive phytochemicals. Overall, this study will help to commercialize those varieties with proper scientific evidences.

中文翻译：

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通过靶向 LC-MS/MS 方法在选定的香味和色素水稻品种中评估营养价值和生物活性植物化学物质的定量分析

印度东北地区 (NER) 的香米 (joha) 和黑米是 40,000 种稻种中的两种，它们因其香气浓郁、药用价值和/或同样值得注意的味道而广受欢迎。进行了生化和基于目标的液相色谱质谱 (LCMS) 分析，以识别和量化这两个品种的选定稻米中的不同植物营养素。生化分析显示，所选黑米 (Chakhao Amubi) 的总酚含量比香米粒 (Kon joha) 高 1.8 倍，总黄酮含量高 2.3 倍。与黑米相比，香米的总淀粉含量显着降低。香米粒中ω-6/ω-3 必需不饱和脂肪酸的健康有益比例明显优于黑米粒。靶向 LC-MS/MS 分析证实了两个样品中都存在谷维素和阿魏酸。在香米中证实存在 4-羟基苯甲酸、芹菜素、三辛酸、燕麦甾醇、香豆素、香豆酸、苯丙氨酸、咖啡酸和α-生育酚，而黑米证实存在原儿茶酸和脱羟基杨梅素. 进一步的定量分析表明，脂质溶血磷脂酰肌醇 (LPI) 16:0、溶血磷脂酰乙醇胺 (LPE) 14:0、溶血磷脂酰胆碱 (LPC) 18:2、LPE 18:2、磷脂酰乙醇胺 (PE) 以及羟基二十二烷酸在香米品种中至少高出三倍；而在Chakhao Amubi，矮牵牛半乳糖苷、LMMPE18:2、PC14:0的含量高于香米粒。总之，包括苯酚、多酚和类黄酮在内的不同植物营养素已被确定为选定水稻品种中的生物活性植物化学物质。实际应用：这项工作将提供有关所研究水稻品种的营养益处的信息。所使用的靶向 LC-MS/MS 分析将提供有关生物活性植物化学物质的一步信息。总的来说，这项研究将有助于利用适当的科学证据将这些品种商业化。这项工作将提供有关所研究水稻品种的营养益处的信息。所使用的靶向 LC-MS/MS 分析将提供有关生物活性植物化学物质的一步信息。总的来说，这项研究将有助于利用适当的科学证据将这些品种商业化。这项工作将提供有关所研究水稻品种的营养益处的信息。所使用的靶向 LC-MS/MS 分析将提供有关生物活性植物化学物质的一步信息。总的来说，这项研究将有助于利用适当的科学证据将这些品种商业化。