The objective of this study was to examine the expression profiles of follistatin (FST ) and its associated molecules (MSTN , INHA , INHBB , INHBA , ACVR2A , and ACVR2B ) in the oviduct of laying hens at 3 hr and 20 hr post‐ovulation (p.o., n  = 5; 35 weeks old), molting (n  = 5; 60 weeks old), and non‐laying (n  = 4; 35–60 weeks old) hens and also to localize the FST by using immunohistochemistry assay. Expression of FST was significantly higher (p  < .05), and MSTN was lower in the uterus of laying hens around 15–20 hr p.o. (during eggshell formation), however, their expressions in the magnum remain unchanged across different physiological stages of hens. FST was mainly expressed in the luminal and glandular epithelium of the uterine tissues, and their expression intensity was highest in laying hens during the eggshell mineralization. There was a relatively increased expression of INHA in the magnum of laying hens around 3 hr p.o. as compared to non‐laying and molting hens. At the same time (3 hr p.o.), there was a significant (p  < .05) decrease in the expression of the INHBB, ACVR2A, and ACV2B . These results indicate that follistatin may regulate the differentiation of uterine luminal and glandular epithelium during eggshell biomineralization.

中文翻译：

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卵泡抑素的表达与蛋鸡输卵管中的卵形成有关。

本研究的目的是检测排卵后 3 小时和 20 小时蛋鸡输卵管中卵泡抑素 ( FST ) 及其相关分子（MSTN、INHA、INHBB、INHBA、ACVR2A和ACVR2B ）的表达谱（ po，n  = 5；35 周龄），蜕皮（n  = 5；60 周龄）和非产蛋（n  = 4；35-60 周龄）母鸡，还通过免疫组织化学测定定位 FST。FST的表达显着更高 ( p  < .05)，而MSTN在 15-20 小时左右（蛋壳形成期间）的产蛋母鸡子宫中较低，然而，它们在大瓶中的表达在母鸡的不同生理阶段保持不变。FST主要表达于子宫组织的管腔和腺体上皮，其表达强度在蛋壳矿化过程中的蛋鸡中最高。与未产蛋和正在蜕皮的母鸡相比，大约 3 小时后，INHA在产蛋母鸡的大腹中的表达相对增加。同时（口服后 3 小时），INHBB、ACVR2A 和 ACV2B 的表达显着 ( p < .05) 下降. 这些结果表明卵泡抑素可能调节蛋壳生物矿化过程中子宫腔和腺上皮的分化。