In this study, not only beta (β)-mannanase fabrication by recombinant Aspergillus sojae AsT3 in solid-state fermentation (SSF), but also the mannooligosaccharide (MOS) production was investigated. Barley husk, wheat bran, rye bran, oat husk, and spent coffee grounds were used as substrates. Plackett–Burman design was used to optimize SSF conditions (temperature, time, inoculation rate, and pH) based on enzyme activity and MOS results. While the highest β-mannanase activity was found to be 983.53 U/mg in rye bran samples, it was found to be 582.59 U/mg, 55.59 U/mg, and 413.50 U/mg for the barley husk, wheat bran, and oat husk, respectively. The highest enzyme activity and MOS production were found to be 255.82 U/mg and 1,079.785 ppm with 7% inoculation rate, 30°C, 7 days and pH 7. Results showed that SSF is suitable for β-mannanase and MOS production due to the evaluation of wastes, lower energy requirement, and suitable environment for growth.

中文翻译：

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从可再生资源上生长的酱油曲霉中表达的烟曲霉生产重组 β-甘露聚糖酶的固态发酵

在这项研究中，不仅通过重组酱油曲霉制造 β (β)-甘露聚糖酶对固态发酵 (SSF) 中的 AsT3 以及甘露寡糖 (MOS) 的生产进行了研究。大麦壳、麦麸、黑麦麸、燕麦壳和废咖啡渣用作基质。Plackett-Burman 设计用于根据酶活性和 MOS 结果优化 SSF 条件（温度、时间、接种率和 pH 值）。虽然在黑麦麸样品中发现最高的 β-甘露聚糖酶活性为 983.53 U/mg，但在大麦壳、麦麸和燕麦中发现它分别为 582.59 U/mg、55.59 U/mg 和 413.50 U/mg壳，分别。发现最高酶活性和 MOS 产量为 255.82 U/mg 和 1,079.785 ppm，接种率为 7%，30°C，7 天，pH 7。结果表明，SSF 适合 β-甘露聚糖酶和 MOS 生产，因为评估废物，降低能源需求，