The present study was conducted to administer a commercial multi‐strain probiotic (MP), Yilibao (Bacillus velezensis:Bacillus cereus:Lactobacillus casei = 2:2:1), in Nile tilapia, Oreochromis niloticus. In terms of aerobic Bacillus spp. counts, we produced five diets containing 0, 0.34, 1.68, 3.36 and 6.72 g/kg of MP dry product (Control, T1, T2, T3, T4). Seven hundred and fifty tilapia juveniles (13.26 ± 0.01 g) distributed into 25 tanks in five replications were fed their diet for eight weeks. Results showed that fish fed T3 and T4 diets displayed significantly higher final body weight and weight gain (p < .05). Further, all MP‐treated fish exhibited remarkably decreased plasma lipid profiles (cholesterol, triglycerides) (p < .05) and fish fed T3 and T4 diets displayed significantly higher plasma myeloperoxidase activity and complement C3 content (p < .05). Additionally, fish fed T3 and T4 diets exhibited significantly promoted total antioxidant capacity, glutathione peroxidase activity, glutathione level and declined malondialdehyde content in the plasma and/or liver (p < .05). Moreover, significantly elevated chymotrypsin activity, villus height and intraepithelial lymphocytes counts were found in fish fed T3 and T4 diets (p < .05). As fish fed T3 and T4 diets surpassed the other treatments in growth, immune–antioxidative status and gut health, the recommended dose for tilapia is 3.36–6.72 g/kg.

中文翻译：

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饮食管理多菌株益生菌对尼罗罗非鱼的生长，血液指标，免疫状态，抗氧化功能和肠道健康的有益影响

本研究是为了管理商用多菌种的益生菌（MP），Yilibao（芽孢杆菌velezensis：蜡状芽孢杆菌：干酪乳杆菌 = 2：2：1），在尼罗罗非鱼，尼罗罗非鱼。就好氧芽孢杆菌而言。计数，我们生产了五种日粮，分别含有0、0.34、1.68、3.36和6.72 g / kg的MP干产品（对照，T1，T2，T3，T4）。将75个罗非鱼幼鱼（13.26±0.01 g）分五次重复分配到25个水箱中，喂食八周。结果表明，喂食T3和T4日粮的鱼显示出更高的最终体重和体重增加（p <.05）。此外，所有经MP处理的鱼均表现出明显降低的血浆脂质谱（胆固醇，甘油三酸酯）（p < .05），并且饲喂T3和T4日粮的鱼显示出明显更高的血浆髓过氧化物酶活性和补体C3含量（p < .05）。此外，以鱼为食的T3和T4日粮在血浆和/或肝脏中的总抗氧化能力，谷胱甘肽过氧化物酶活性，谷胱甘肽水平和丙二醛含量显着提高（p < .05）。此外，在喂食T3和T4日粮的鱼中，发现胰凝乳蛋白酶活性，绒毛高度和上皮内淋巴细胞计数显着升高（p <.05）。随着用T3和T4饲料喂养的鱼在生长，免疫-抗氧化状态和肠道健康方面超过其他治疗方法，罗非鱼的推荐剂量为3.36–6.72 g / kg。