Abstract This study examines the effects of hot water dipping on wound healing of harvested potato tubers and to explore partial mechanisms. The potato ‘cv. Longshu No. 7′ was used as materials, which were artificially wounded and then dipped into 45 °C hot water for 10 min. The wounded tubers were stored at ambient temperature (20−25 °C, RH 70–80%) in dark for wound healing. The weight loss of wounded tubers and the disease index of wounded tubers inoculated with Fusarium sulphureum were determined. The accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at wound sites were observed. The activity of phenylalanine ammonia lyase, peroxidase activity, the content of H2O2, total phenols, flavonoids and lignin were also measured. We found that hot water dipping effectively reduced the weight loss and the disease index of wounded potato tubers. The weight loss of treated tubers was 45.1% lower than the control on the 14 d of healing, and the disease index of treated tubers was 18.2% lower than the control on the 21 d of healing. Hot water dipping significantly stimulated the accumulation of suberin poly phenolic, suberin poly aliphatic and lignin at wound sites. The thickness of suberin poly phenolic, suberin poly aliphatic and lignin cell layers of treated tubers were 28.9%, 23.2% and 18.9% higher than the control on the 21 d of healing, respectively. Moreover, hot water dipping significantly enhanced the activity of phenylalanine ammonia lyase and peroxidase. Meanwhile, the H2O2 content, total phenols, flavonoids and lignin were also significantly promoted at wound sites by hot water dipping treatment during healing. These results suggested that hot water dipping promotes wound healing of potato tubers by activating phenylpropanoid metabolism, increasing H2O2 content and peroxidase activity, promoting the suberin and lignin accumulation at wound sites and decreasing the weight loss and the disease index of tubers during healing.

中文翻译：

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热水浸泡刺激马铃薯块茎伤口愈合

摘要 本研究考察了热水浸泡对收获的马铃薯块茎伤口愈合的影响，并探讨了部分机制。马铃薯'cv。以龙舒7′号为原料，人工创面后浸入45℃热水中10分钟。受伤的块茎在环境温度（20-25°C，RH 70-80%）下储存在黑暗中用于伤口愈合。测定了接种硫磺镰刀菌的受伤块茎的失重和受伤块茎的疾病指数。观察到木栓质多酚、木栓质多脂族和木质素在伤口部位的积累。还测定了苯丙氨酸解氨酶的活性、过氧化物酶的活性、H2O2 的含量、总酚、黄酮类和木质素的含量。我们发现热水浸泡有效地减少了受伤的马铃薯块茎的体重减轻和疾病指数。治疗14 d块茎失重比对照降低45.1%，治疗21 d块茎疾病指数比对照降低18.2%。热水浸泡显着刺激了木栓质多酚、木栓质多脂肪族和木质素在伤口部位的积累。处理后块茎的木栓质多酚、木栓质多脂族和木质素细胞层厚度在愈合第21天分别比对照高28.9%、23.2%和18.9%。此外，热水浸泡显着增强了苯丙氨酸解氨酶和过氧化物酶的活性。同时，H2O2 含量、总酚、在愈合过程中，热水浸渍处理也显着促进了伤口部位的黄酮类化合物和木质素。这些结果表明，热水浸渍通过激活苯丙烷代谢，增加过氧化物酶的含量和过氧化物酶活性，促进伤口部位木栓质和木质素的积累，降低愈合过程中块茎的体重减轻和疾病指数，从而促进马铃薯块茎的伤口愈合。