Molecular characterization of a voltage-gated calcium channel and its potential role in the acaricidal action of scopoletin against Tetranychus cinnabarinus,Pesticide Biochemistry and Physiology

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Molecular characterization of a voltage-gated calcium channel and its potential role in the acaricidal action of scopoletin against Tetranychus cinnabarinus
Pesticide Biochemistry and Physiology ( IF 4.2 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.pestbp.2020.104618
Xiao-Feng Ma ₁ , Yuan-Yuan Zhang ₁ , Fu-You Guo ₁ , Jin-Xiang Luo ₁ , Wei Ding ₂ , Yong-Qiang Zhang ₃

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The carmine spider mite, Tetranychus cinnabarinus (Boisduval), is a polyphagous agricultural pest with an extensive host plant range. Scopoletin is a promising acaricidal compound whose acaricidal mechanism may occur by disrupting intracellular Ca2+ homeostasis and calcium signaling pathways. However, the underlying mechanism of scopoletin for specific target locations of T. cinnabarinus remains unclear. In this study, a full-length cDNA of the L-type voltage-gated calcium channel (TcLTCC) subunit gene from T. cinnabarinus was cloned and characterized. The expression pattern of the TcLTCC gene in all developmental stages of T. cinnabarinus was analyzed. The gene was highly expressed in larval and nymphal stages and was significantly upregulated after treatment with scopoletin. Knocking down the TcLTCC transcript reduced the sensitivity of T. cinnabarinus to scopoletin. Homology modeling and molecular docking were also conducted. The interaction between scopoletin and TcLTCC showed that scopoletin inserted into the cavity bound to the site of the TcLTCC protein by the driving force of hydrogen bonding. This study provides insights into the mechanism by which scopoletin interacts with TcLTCC. Results can improve the understanding of the toxicity of scopoletin to T. cinnabarinus and provide valuable information for the design of new LTCC inhibitors.

中文翻译：

电压门控钙通道的分子特征及其在东莨菪碱对朱砂叶螨杀螨作用中的潜在作用

胭脂红叶螨 Tetranychus cinnabarinus (Boisduval) 是一种多食性农业害虫，寄主植物范围广泛。Scopoletin 是一种很有前途的杀螨剂化合物，其杀螨机制可能通过破坏细胞内 Ca2+ 稳态和钙信号通路来实现。然而，东莨菪碱对朱砂特定目标位置的潜在机制仍不清楚。在这项研究中，来自朱砂的 L 型电压门控钙通道 (TcLTCC) 亚基基因的全长 cDNA 被克隆和表征。分析了 TcLTCC 基因在 T. cinnabarinus 的所有发育阶段的表达模式。该基因在幼虫和若虫阶段高表达，用东莨菪碱处理后显着上调。敲低 TcLTCC 转录本会降低 T. 朱砂到东莨菪碱。还进行了同源建模和分子对接。东莨菪碱与TcLTCC的相互作用表明，东莨菪碱插入腔内，通过氢键作用与TcLTCC蛋白的位点结合。这项研究提供了对东莨菪碱与 TcLTCC 相互作用机制的见解。结果可以提高对东莨菪碱对朱砂毒性的认识，并为设计新的 LTCC 抑制剂提供有价值的信息。这项研究提供了对东莨菪碱与 TcLTCC 相互作用机制的见解。结果可以提高对东莨菪碱对朱砂毒性的认识，并为设计新的 LTCC 抑制剂提供有价值的信息。这项研究提供了对东莨菪碱与 TcLTCC 相互作用机制的见解。结果可以提高对东莨菪碱对朱砂毒性的认识，并为设计新的 LTCC 抑制剂提供有价值的信息。

更新日期：2020-09-01

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