The application of rhizobial cells for improving pigeon pea fitness has been practiced for ages. As cell-based approaches have limitations, the focus is on the usage of cell-free formulations. In the present study exopolysaccharide (EPS) producing, plant health promoting Bradyrhizobium sp. IC-4059 was monitored for its biocontrol potential against Fusarium udum. Strain IC-4059, its EPS, and supernatant antagonized F.udum by 52.6%, 20.5%, and 48.1%, respectively in comparison to control. Diverse formulations prepared using EPS, supernatant, and strain IC-4059 cells were: EPS based, supernatant based, IC-4059 liquid culture (rhizobia based), dual combination (IC-4059 cells + supernatant; IC-4059 cells + EPS; EPS + supernatant) and triple combination (IC-4059 cells + supernatant + EPS). The potency of these bioformulations was observed under in planta tubes following in vivo pot study, both in F.udum infested and non-infested sets taking pigeon pea cultivar UPAS-120 as a model crop. The study highlighted that seeds receiving triple combination exhibited significantly higher plant growth attributes, nodule enhancing, and disease suppressing activity in comparison to individual application of Bradyrhizobium. Antifungal compounds were analyzed by a metabolomic approach using UPLC-MS. Thus, the study disseminates the tripartite role of triple formulation as a plant growth stimulator, nodule enhancer, and disease suppressor, both under in vitro and in vivo conditions in the presence of phytopathogen F. udum.

根瘤菌细胞在改善木豆健康方面的应用已有很长时间了。由于基于细胞的方法存在局限性，因此重点在于无细胞制剂的使用。在本研究中，胞外多糖（EPS）的产生，促进植物健康的Bradyrhizobium sp。监测IC-4059对镰刀菌的生防潜力。IC-4059菌株，其EPS和上清拮抗金缕梅与对照组相比分别增加了52.6％，20.5％和48.1％。使用EPS，上清液和IC-4059细胞株制备的多种制剂为：EPS，基于上清液，IC-4059液体培养物（根瘤菌），双重组合（IC-4059细胞+上清液; IC-4059细胞+ EPS; EPS） +上清液）和三重组合（IC-4059细胞+上清液+ EPS）。在体内盆栽研究之后，在植物足底试管中均观察到了这些生物制剂的效价，均在乌。中以木豆品种UPAS-120为模型作物的被侵染和未侵染的作物。研究强调，与单独施用缓生根瘤菌相比，接受三联组合的种子表现出显着更高的植物生长特性，结节增强和抑制病害的活性。使用UPLC-MS通过代谢组学方法分析了抗真菌化合物。因此，本研究传播三重制剂的三方的角色作为植物生长刺激剂，结节增强剂和疾病抑制器，两者下在体外和体内在植物病原体的存在条件F. udum。