In this study, two accurate, precise, selective and sensitive methods were developed for determining aflatoxin M1 (AFM1) in infant formula milk using immunoaffinity column clean-up followed by high performance liquid chromatography (HPLC) with fluorescence detection. The validated methods were used for determination of AFM1 in 29 samples of 6 different infant formula milk brands and the risk of AFM1 in infants aged zero to 6 months old was assessed using cancer risk, Margin of Exposure (MOE) and Hazard Index (HI). Only one sample (3.4%) was contaminated with AFM1. Although the results showed that MOE values for the mean and median exposure to AFM1 was <10,000 in infants, the additional cancer risk due to mean and median exposure to AFM1 in infant <6 months were 0.00010 and 0.00012 additional cases per year per 10⁵ individuals, respectively, which indicates no health concern. In addition, HI values for the mean and median exposure to AFM1 for infants were quite below one which indicates no health concern. To the best of our knowledge, this is the first report on risk assessment of AFM1 in infant formula milk consumed by Iranian infants <6 months old, presenting a low risk for the evaluated groups.

在这项研究中，开发了两种准确，精确，选择性和灵敏的方法，分别采用免疫亲和柱净化和高效液相色谱（HPLC）和荧光检测来测定婴儿配方奶中的黄曲霉毒素M1（AFM1）。经验证的方法用于测定6个不同婴儿配方奶粉品牌的29个样品中的AFM1，并使用癌症风险，暴露率（MOE）和危险指数（HI）评估了0至6个月大的婴儿中AFM1的风险。 。只有一个样品（3.4％）被AFM1污染。尽管结果表明，婴儿的AFM1平均暴露量和中位数的MOE值小于10,000，但每6 ⁵岁以下婴儿由于AFM1的平均暴露量和中位数，每年的额外癌症风险为0.00010和0.00012分别表示没有健康问题。此外，婴儿的AFM1暴露的平均值和中位数的HI值远低于1，表明没有健康问题。据我们所知，这是关于<6个月大的伊朗婴儿食用的婴儿配方奶粉中AFM1风险评估的第一份报告，对评估组的风险较低。